Distribution of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Mitotypes in Commercial Cotton Fields in the Punjab Province of Pakistan

Abstract

The Bemisia tabaci (Gennadius) (Hemiptera: Aleyroridae) sibling species group is comprised of genetic variants defined by biological differences or a fragment of mitochondrial cytochrome oxidase I gene sequence (mitotype) that allows for phylogeographic affiliation. Some mitotypes may cause damage to crop plants by feeding and transmission of plant viruses. In Pakistan, cotton-vegetable agroecosystems are vulnerable to whitefly-transmitted virus (genus Begomovirus; family Geminiviridae) infection. The identity and distribution of the whitefly B. tabaci mitotypes associated with the cotton crop were studied in 8 districts in the Punjab Province from 2014 to 2016. Phylogenetic analysis of the 3(sic)-fragment of the mitochondrial cytochrome oxidase I gene indicated the predominant haplotypes belonged to the Asia II-1 mitotype, with pairwise distances ranging from 0.15 to 3.2%. Pairwise distances showed that B. tabaci haplotype diversity varied by district, with the Khanewal harboring the highest divergence at 1.37%, compared to the lowest at 0.50% in the Dera Ghazi Khan district. The median-joining network analysis showed genetic expansion, or a 'recovery' trend, following the declining genetic diversity that occurred during the late 1990s to the early 2000s. The Asia II-1 mitotype group was the predominant whitefly vector species in Punjab Province. The haplotype network provides documentation of continued genetic expansion among the B. tabaci populations in the Punjab, which is consistent with previously reported trends among whiteflies sampled in the same or nearby districts from 2012 to 2014. Genetic expansion varied among districts and could be explained by factors unique to each district, i.e., management practices that influence B. tabaci mitotype composition, whitefly susceptibility to cotton leaf curl disease complex, and cotton genotype