Design, Applications and Characterization of All-Reflective Multi-Modal Microscopes
AuthorCromey, Benjamin Matthew
AdvisorKieu, Khanh Q.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
EmbargoRelease after 05/27/2021
AbstractSince the first demonstration of multiphoton microscopy in 1990, the multiphoton microscope has found broad use in science, predominantly in the biological sciences. Much work has been done demonstrating the usefulness of this microscopy technique. Many different companies and lab groups have created their own microscope systems, each with advantages and disadvantages. Predominantly, however, the microscopes have similar design forms, depending on refractive optics to control the ultrafast laser that powers the microscope. In this dissertation, two all-reflective microscope designs are presented to demonstrate their key advantages for multiphoton microscopy; namely, the removal of the effects of dispersion such as chromatic aberration and pulse spreading. The all-reflective architecture also easily supports multiple modes of microscopy, enabling a compact and versatile new instrument. In addition, the design of a new laser that takes advantage of this all-reflective architecture is presented. Finally, two applications of multiphoton microscopy are presented: the study of pancreatic cancer margins to increase the survival rate of patients in surgery, and the study of gems and minerals, a world-first new application of multiphoton microscopy.
Degree ProgramGraduate College