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dc.contributor.authorFontenele, Rafaela S
dc.contributor.authorSalywon, Andrew M
dc.contributor.authorMajure, Lucas C
dc.contributor.authorCobb, Ilaria N
dc.contributor.authorBhaskara, Amulya
dc.contributor.authorAvalos-Calleros, Jesús A
dc.contributor.authorArgüello-Astorga, Gerardo R
dc.contributor.authorSchmidlin, Kara
dc.contributor.authorKhalifeh, Anthony
dc.contributor.authorSmith, Kendal
dc.contributor.authorSchreck, Joshua
dc.contributor.authorLund, Michael C
dc.contributor.authorKöhler, Matias
dc.contributor.authorWojciechowski, Martin F
dc.contributor.authorHodgson, Wendy C
dc.contributor.authorPuente-Martinez, Raul
dc.contributor.authorVan Doorslaer, Koenraad
dc.contributor.authorKumari, Safaa
dc.contributor.authorVernière, Christian
dc.contributor.authorFilloux, Denis
dc.contributor.authorRoumagnac, Philippe
dc.contributor.authorLefeuvre, Pierre
dc.contributor.authorRibeiro, Simone G
dc.contributor.authorKraberger, Simona
dc.contributor.authorMartin, Darren P
dc.contributor.authorVarsani, Arvind
dc.date.accessioned2020-07-07T17:39:13Z
dc.date.available2020-07-07T17:39:13Z
dc.date.issued2020-04-03
dc.identifier.citationFontenele, R.S.; Salywon, A.M.; Majure, L.C.; Cobb, I.N.; Bhaskara, A.; Avalos-Calleros, J.A.; Argüello-Astorga, G.R.; Schmidlin, K.; Khalifeh, A.; Smith, K.; Schreck, J.; Lund, M.C.; Köhler, M.; Wojciechowski, M.F.; Hodgson, W.C.; Puente-Martinez, R.; Van Doorslaer, K.; Kumari, S.; Vernière, C.; Filloux, D.; Roumagnac, P.; Lefeuvre, P.; Ribeiro, S.G.; Kraberger, S.; Martin, D.P.; Varsani, A. A Novel Divergent Geminivirus Identified in Asymptomatic New World Cactaceae Plants. Viruses 2020, 12, 398.en_US
dc.identifier.issn1999-4915
dc.identifier.pmid32260283
dc.identifier.doi10.3390/v12040398
dc.identifier.urihttp://hdl.handle.net/10150/641813
dc.description.abstractCactaceae comprise a diverse and iconic group of flowering plants which are almost exclusively indigenous to the New World. The wide variety of growth forms found amongst the cacti have led to the trafficking of many species throughout the world as ornamentals. Despite the evolution and physiological properties of these plants having been extensively studied, little research has focused on cactus-associated viral communities. While only single-stranded RNA viruses had ever been reported in cacti, here we report the discovery of cactus-infecting single-stranded DNA viruses. These viruses all apparently belong to a single divergent species of the family Geminiviridae and have been tentatively named Opuntia virus 1 (OpV1). A total of 79 apparently complete OpV1 genomes were recovered from 31 different cactus plants (belonging to 20 different cactus species from both the Cactoideae and Opuntioideae clades) and from nine cactus-feeding cochineal insects (Dactylopius sp.) sampled in the USA and Mexico. These 79 OpV1 genomes all share > 78.4% nucleotide identity with one another and < 64.9% identity with previously characterized geminiviruses. Collectively, the OpV1 genomes display evidence of frequent recombination, with some genomes displaying up to five recombinant regions. In one case, recombinant regions span similar to 40% of the genome. We demonstrate that an infectious clone of an OpV1 genome can replicate in Nicotiana benthamiana and Opuntia microdasys. In addition to expanding the inventory of viruses that are known to infect cacti, the OpV1 group is so distantly related to other known geminiviruses that it likely represents a new geminivirus genus. It remains to be determined whether, like its cactus hosts, its geographical distribution spans the globe.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsCopyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectCactoideaeen_US
dc.subjectOpuntioideaeen_US
dc.subjectcochineal insectsen_US
dc.subjectGeminivirusen_US
dc.subjectssDNA virusen_US
dc.titleA Novel Divergent Geminivirus Identified in Asymptomatic New World Cactaceae Plantsen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Sch Anim & Comparat Biomed Sci, Dept Immunobiol, Inst BIO5en_US
dc.contributor.departmentUniv Arizona, UA Canc Ctren_US
dc.identifier.journalVIRUSES-BASELen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleViruses
dc.source.volume12
dc.source.issue4
refterms.dateFOA2020-07-07T17:39:14Z
dc.source.countrySwitzerland


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