Human Cytomegalovirus Infection Suppresses CD34(+) Progenitor Cell Engraftment in Humanized Mice
AuthorCrawford, Lindsey B
Streblow, Daniel N
Yurochko, Andrew D
Goodrum, Felicia D
Nelson, Jay A
AffiliationUniv Arizona, BIO5 Inst, Dept Immunobiol
hematopoietic stem cell transplant
MetadataShow full item record
CitationCrawford, L.B.; Tempel, R.; Streblow, D.N.; Yurochko, A.D.; Goodrum, F.D.; Nelson, J.A.; Caposio, P. Human Cytomegalovirus Infection Suppresses CD34+ Progenitor Cell Engraftment in Humanized Mice. Microorganisms 2020, 8, 525.
RightsCopyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractHuman cytomegalovirus (HCMV) infection is a serious complication in hematopoietic stem cell transplant (HSCT) recipients due to virus-induced myelosuppression and impairment of stem cell engraftment. Despite the clear clinical link between myelosuppression and HCMV infection, little is known about the mechanism(s) by which the virus inhibits normal hematopoiesis because of the strict species specificity and the lack of surrogate animal models. In this study, we developed a novel humanized mouse model system that recapitulates the HCMV-mediated engraftment failure after hematopoietic cell transplantation. We observed significant alterations in the hematopoietic populations in peripheral lymphoid tissues following engraftment of a subset of HCMV+ CD34(+) hematopoietic progenitor cells (HPCs) within the transplant, suggesting that a small proportion of HCMV-infected CD34(+) HPCs can profoundly affect HPC differentiation in the bone marrow microenvironment. This model will be instrumental to gain insight into the fundamental mechanisms of HCMV myelosuppression after HSCT and provides a platform to assess novel treatment strategies.
NoteOpen access journal
VersionFinal published version
- Human Cytomegalovirus US28 Ligand Binding Activity Is Required for Latency in CD34<sup>+</sup> Hematopoietic Progenitor Cells and Humanized NSG Mice.
- Authors: Crawford LB, Caposio P, Kreklywich C, Pham AH, Hancock MH, Jones TA, Smith PP, Yurochko AD, Nelson JA, Streblow DN
- Issue date: 2019 Aug 20
- CD34<sup>+</sup> Hematopoietic Progenitor Cell Subsets Exhibit Differential Ability To Maintain Human Cytomegalovirus Latency and Persistence.
- Authors: Crawford LB, Hancock MH, Struthers HM, Streblow DN, Yurochko AD, Caposio P, Goodrum FD, Nelson JA
- Issue date: 2021 Jan 13
- Human Cytomegalovirus Encodes a Novel FLT3 Receptor Ligand Necessary for Hematopoietic Cell Differentiation and Viral Reactivation.
- Authors: Crawford LB, Kim JH, Collins-McMillen D, Lee BJ, Landais I, Held C, Nelson JA, Yurochko AD, Caposio P
- Issue date: 2018 Apr 24
- Human Cytomegalovirus miRNAs Regulate TGF-β to Mediate Myelosuppression while Maintaining Viral Latency in CD34<sup>+</sup> Hematopoietic Progenitor Cells.
- Authors: Hancock MH, Crawford LB, Pham AH, Mitchell J, Struthers HM, Yurochko AD, Caposio P, Nelson JA
- Issue date: 2020 Jan 8
- Human Cytomegalovirus Requires Epidermal Growth Factor Receptor Signaling To Enter and Initiate the Early Steps in the Establishment of Latency in CD34<sup>+</sup> Human Progenitor Cells.
- Authors: Kim JH, Collins-McMillen D, Buehler JC, Goodrum FD, Yurochko AD
- Issue date: 2017 Mar 1