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    Technical note: A comparison of techniques for extracting monoterpenoids from Juniperus (Cupressaceae) species

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    Author
    Owens, M. K.
    Straka, E. J.
    Carroll, C. J.
    Taylor, C. A.
    Issue Date
    1998-09-01
    Keywords
    distillation
    hexane
    gas chromatography
    steam distallation
    extraction
    Juniperus ashei
    Juniperus pinchotii
    monoterpenoids
    conifer needles
    duration
    Texas
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    Citation
    Owens, M. K., Straka, E. J., Carroll, C. J., & Taylor, C. A. (1998). A comparison of techniques for extracting monoterpenoids from Juniperus (Cupressaceae) species. Journal of Range Management, 51(5), 540-544.
    Publisher
    Society for Range Management
    Journal
    Journal of Range Management
    URI
    http://hdl.handle.net/10150/644029
    DOI
    10.2307/4003372
    Additional Links
    https://rangelands.org/
    Abstract
    Concentration and composition of monoterpenoids in plant tissue affects a variety of environmental and ecological issues such as plant defenses, plant classification and phytotoxicity. Developing the techniques for extracting and estimating the concentration and composition of monoterpenoids must be species-specific because monoterpenoid storage location varies between species. Ashe juniper (Juniperus ashei Buchholz) and redberry juniper (J. pinchotti Sudw) are 2 co-occurring species which differ in palatability and preference. The objective of this study was to determine which of 2 common extraction techniques provided the best estimate of the concentration and composition of monoterpenoids in mature plant tissue. Two extraction techniques were tested by soaking crushed juniper needles in hexane solvent for 6, 12, 18 and 24 hours or by steam distilling samples for 2, 4, 6, or 8 hours. The extracts were analyzed by using 2 different analytical columns in separate gas chromatographs. The hexane solvent soak, regardless of time in the solvent yielded a lower total concentration and a decreased compositional diversity of monoterpenoids compared to the steam distillation technique. An 8-hour steam distillation yielded the greatest concentration and composition of monoterpenoids. Both types of analytical columns resulted in similar estimates of monoterpenoid concentrations and composition.
    Type
    text
    Article
    Language
    en
    ISSN
    0022-409X
    ae974a485f413a2113503eed53cd6c53
    10.2307/4003372
    Scopus Count
    Collections
    Journal of Range Management, Volume 51, Number 5 (September 1998)

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