Technical note: A comparison of techniques for extracting monoterpenoids from Juniperus (Cupressaceae) species
Issue Date
1998-09-01Keywords
distillationhexane
gas chromatography
steam distallation
extraction
Juniperus ashei
Juniperus pinchotii
monoterpenoids
conifer needles
duration
Texas
Metadata
Show full item recordCitation
Owens, M. K., Straka, E. J., Carroll, C. J., & Taylor, C. A. (1998). A comparison of techniques for extracting monoterpenoids from Juniperus (Cupressaceae) species. Journal of Range Management, 51(5), 540-544.Publisher
Society for Range ManagementJournal
Journal of Range ManagementDOI
10.2307/4003372Additional Links
https://rangelands.org/Abstract
Concentration and composition of monoterpenoids in plant tissue affects a variety of environmental and ecological issues such as plant defenses, plant classification and phytotoxicity. Developing the techniques for extracting and estimating the concentration and composition of monoterpenoids must be species-specific because monoterpenoid storage location varies between species. Ashe juniper (Juniperus ashei Buchholz) and redberry juniper (J. pinchotti Sudw) are 2 co-occurring species which differ in palatability and preference. The objective of this study was to determine which of 2 common extraction techniques provided the best estimate of the concentration and composition of monoterpenoids in mature plant tissue. Two extraction techniques were tested by soaking crushed juniper needles in hexane solvent for 6, 12, 18 and 24 hours or by steam distilling samples for 2, 4, 6, or 8 hours. The extracts were analyzed by using 2 different analytical columns in separate gas chromatographs. The hexane solvent soak, regardless of time in the solvent yielded a lower total concentration and a decreased compositional diversity of monoterpenoids compared to the steam distillation technique. An 8-hour steam distillation yielded the greatest concentration and composition of monoterpenoids. Both types of analytical columns resulted in similar estimates of monoterpenoid concentrations and composition.Type
textArticle
Language
enISSN
0022-409Xae974a485f413a2113503eed53cd6c53
10.2307/4003372