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    • Journal of Range Management, Volume 50 (1997)
    • Journal of Range Management, Volume 50, Number 4 (July 1997)
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    In vitro regeneration of fourwing saltbush [Atriplex canescens (Pursh) Nutt.]

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    Author
    Mei, B.
    No, E. G.
    McWilliams, E. L.
    Gould, J. H.
    Newton, R. J.
    Issue Date
    1997-07-01
    Keywords
    regenerative ability
    vegetative propagation
    organogenesis
    tissue culture
    shoots
    Atriplex canescens
    
    Metadata
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    Citation
    Mei, B., No, E. G., McWilliams, E. L., Gould, J. H., & Newton, R. J. (1997). In vitro regeneration of fourwing saltbush [Atriplex canescens (Pursh) Nutt.]. Journal of Range Management, 50(4), 413-418.
    Publisher
    Society for Range Management
    Journal
    Journal of Range Management
    URI
    http://hdl.handle.net/10150/644123
    DOI
    10.2307/4003309
    Additional Links
    https://rangelands.org/
    Abstract
    A successful, in vitro regeneration system for fourwing saltbush [Atriplex canescens (Pursh) Nutt.] has potential use for mass propagation and genetic engineering of this important range species. This system could be used as a vehicle for gene transfer and for genetic modification of forage quality, stress tolerance, and biomass. The objectives were to develop an in vitro system for shoot production from both leaf discs and axillary buds, and induce root production. Shoot organogenesis (265 shoots) from leaf disc explants was accomplished at rates of 12.3 shoots/disc or 1.7 shoots/mm2 of leaf disc explants. Root organogenesis was induced in 63% (168) of the shoots, using indolebutyric acid (IBA, 0.5 mg liter-1) and gibberellic acid (GA3, 0.1 mg-1 liter) in a Murashige and Skoog (MS) medium. Roots were also induced on shoots placed in soil. Survival of both shoots and plantlets transferred to soil was 65%. Plantlets transferred to a hydroponic solution were twice the height of plantlets grown in soil. For axillary bud multiplication, the number of shoots generated ranged from 0.7 to 9.1 per explant. Roots were induced on 63% of the shoots, and 58% survived transfer to soil. The successful production of plantlets using both leaf-disc and axillary-bud methods demonstrates that this important range species can be mass propagated in vitro.
    Type
    text
    Article
    Language
    en
    ISSN
    0022-409X
    ae974a485f413a2113503eed53cd6c53
    10.2307/4003309
    Scopus Count
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    Journal of Range Management, Volume 50, Number 4 (July 1997)

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