Effects of incubation time and sodium sulfite upon in-vitro digestibility estimates and sample filtering time
Issue Date
1995-09-01Keywords
wheat strawsodium sulfite
filterability
laboratory techniques
Panicum coloratum
alfalfa
alfalfa hay
organic matter
lignin
estimation
duration
fiber content
hay
in vitro digestibility
grasses
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Show full item recordCitation
Hunt, J., Pinchak, W., & Hutcheson, D. A. V. I. D. (1995). Effects of incubation time and sodium sulfite upon in-vitro digestibility estimates and sample filtering time. Journal of Range Management, 48(5), 451-454.Publisher
Society for Range ManagementJournal
Journal of Range ManagementDOI
10.2307/4002251Additional Links
https://rangelands.org/Abstract
We conducted 2 experiments to quantify the effects of incubation time, filtering method, forage type, and associated interactions on the precision and accuracy of in-vitro digestibility as estimates of in-vivo digestibility. Experiment I used 10 incubation times and alfalfa (Medicago sativa L.), kleingrass (Panicum coloratum L.), prairie grass, and wheat straw (Triticum aestivum L.) hays to determine whether a single incubation time should be employed to estimate digestibility of a variety of forages. Additionally, 2 second stage neutral detergent extraction methods were evaluated to determine sodium sulfite effect on fiber recovery and filter time. An interaction existed between incubation time and in-vitro estimates of digestibility. The use of sodium sulfite increased (P<0.05) digestibility estimates (1.3 units) across alI hays and decreased filtering times by as much as 9.5 min/sample. Esperiment II utilized 3 hays (alfalfa, kleingrass, and wheat straw), 4 incubation times and 4 neutral detergent extraction methods in an effort to isolate where the changes in neutral detergent fiber (NDF) estimates due to sodium sulfite occurred and if a method could be developed to maximize filtering speed without compromising the accuracy of digestibility estimates. Use of sodium sulfite in the rinse mater did not affect apparent NDF recovery and decreased filtering time by approximately 10 min. when compared to no sulfite additions. Results of this study confirm previous observations that a single incubation period should not be used to estimate in-vivo digestibility. Addition of sodium sulfite to the rinse water provides a viable means to decrease sample anaIysis time without jeopardizing the accuracy of digestible NDF estimates.Type
textArticle
Language
enISSN
0022-409Xae974a485f413a2113503eed53cd6c53
10.2307/4002251