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    Effects of incubation time and sodium sulfite upon in-vitro digestibility estimates and sample filtering time

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    Author
    Hunt, J.
    Pinchak, W.
    Hutcheson, D.
    Issue Date
    1995-09-01
    Keywords
    wheat straw
    sodium sulfite
    filterability
    laboratory techniques
    Panicum coloratum
    alfalfa
    alfalfa hay
    organic matter
    lignin
    estimation
    duration
    fiber content
    hay
    in vitro digestibility
    grasses
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    Citation
    Hunt, J., Pinchak, W., & Hutcheson, D. A. V. I. D. (1995). Effects of incubation time and sodium sulfite upon in-vitro digestibility estimates and sample filtering time. Journal of Range Management, 48(5), 451-454.
    Publisher
    Society for Range Management
    Journal
    Journal of Range Management
    URI
    http://hdl.handle.net/10150/644241
    DOI
    10.2307/4002251
    Additional Links
    https://rangelands.org/
    Abstract
    We conducted 2 experiments to quantify the effects of incubation time, filtering method, forage type, and associated interactions on the precision and accuracy of in-vitro digestibility as estimates of in-vivo digestibility. Experiment I used 10 incubation times and alfalfa (Medicago sativa L.), kleingrass (Panicum coloratum L.), prairie grass, and wheat straw (Triticum aestivum L.) hays to determine whether a single incubation time should be employed to estimate digestibility of a variety of forages. Additionally, 2 second stage neutral detergent extraction methods were evaluated to determine sodium sulfite effect on fiber recovery and filter time. An interaction existed between incubation time and in-vitro estimates of digestibility. The use of sodium sulfite increased (P<0.05) digestibility estimates (1.3 units) across alI hays and decreased filtering times by as much as 9.5 min/sample. Esperiment II utilized 3 hays (alfalfa, kleingrass, and wheat straw), 4 incubation times and 4 neutral detergent extraction methods in an effort to isolate where the changes in neutral detergent fiber (NDF) estimates due to sodium sulfite occurred and if a method could be developed to maximize filtering speed without compromising the accuracy of digestibility estimates. Use of sodium sulfite in the rinse mater did not affect apparent NDF recovery and decreased filtering time by approximately 10 min. when compared to no sulfite additions. Results of this study confirm previous observations that a single incubation period should not be used to estimate in-vivo digestibility. Addition of sodium sulfite to the rinse water provides a viable means to decrease sample anaIysis time without jeopardizing the accuracy of digestible NDF estimates.
    Type
    text
    Article
    Language
    en
    ISSN
    0022-409X
    ae974a485f413a2113503eed53cd6c53
    10.2307/4002251
    Scopus Count
    Collections
    Journal of Range Management, Volume 48, Number 5 (September 1995)

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