Characterization of a cell death-inducing endonuclease-like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae)
AffiliationUniv Arizona, Dept Entomol
MetadataShow full item record
PublisherJOHN WILEY & SONS LTD
CitationWang, J., Yan, Z., Xiao, S., Wang, B., Fang, Q., Schlenke, T., & Ye, G. (2020). Characterization of a cell death‐inducing endonuclease‐like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Pest Management Science.
JournalPEST MANAGEMENT SCIENCE
RightsCopyright © 2020 Society of Chemical Industry.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractBACKGROUND Parasitoid wasps are valuable natural enemies for controlling pests. To ensure successful parasitism, these wasps inject venoms along with their eggs that are deposited either into or on their hosts. Parasitoid venoms regulate host behaviors, development, metabolism and immune responses. Pteromalus puparum is a pupal endoparasitoid that parasitizes a number of butterflies, including the worldwide pest cabbage butterfly, Pieris rapae. Venom from P. puparum has a variety of effects on host hemocytes, including alteration of absolute and relative hemocyte counts, and inhibition of hemocyte spreading and encapsulation. In particular, P. puparum venom causes hemocyte cell death in vivo and in vitro. RESULTS Using assay-guided chromatography, a cell death-inducing venom fraction was identified and defined as P. puparum endonuclease-like venom protein (PpENVP). It belongs to the DNA/RNA nonspecific endonuclease family, which contains two conserved endonuclease activation sites. We analyzed its expression profiles and demonstrated that PpENVP inhibits gene expression in transfected cells relying on two activation sites. However, RNA interference of PpENVP did not significantly reduce P. puparum venom cytotoxicity, suggesting that PpENVP may not be the sole cytotoxic factor present. CONCLUSION Our results provide novel insight into the function of the P. puparum venom cocktail and identify a promising insecticide candidate endonuclease that targets insect hemocytes.
Note12 month embargo; first published 16 July 2020
VersionFinal accepted manuscript
- Parasitism of Pieris rapae (Lepidoptera: Pieridae) by a pupal endoparasitoid, Pteromalus puparum (Hymenoptera: Pteromalidae): effects of parasitization and venom on host hemocytes.
- Authors: Cai J, Ye GY, Hu C
- Issue date: 2004 Apr
- Expression of immune-response genes in lepidopteran host is suppressed by venom from an endoparasitoid, Pteromalus puparum.
- Authors: Fang Q, Wang L, Zhu J, Li Y, Song Q, Stanley DW, Akhtar ZR, Ye G
- Issue date: 2010 Sep 2
- Venom α-amylase of the endoparasitic wasp Pteromalus puparum influences host metabolism.
- Authors: Wang B, Ren C, Yang L, Fang Q, Song Q, Ye G
- Issue date: 2020 Jun
- Lipidomics reveals how the endoparasitoid wasp Pteromalus puparum manipulates host energy stores for its young.
- Authors: Wang J, Jin H, Schlenke T, Yang Y, Wang F, Yao H, Fang Q, Ye G
- Issue date: 2020 Sep
- Isolation and characterization of an immunosuppressive protein from venom of the pupa-specific endoparasitoid Pteromalus puparum.
- Authors: Wu ML, Ye GY, Zhu JY, Chen XX, Hu C
- Issue date: 2008 Oct