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    Characterization of a cell death-inducing endonuclease-like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

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    Author
    Wang, Jiale
    Yan, Zhichao
    Xiao, Shan
    Wang, Beibei
    Fang, Qi
    Schlenke, Todd
    Ye, Gongyin
    Affiliation
    Univ Arizona, Dept Entomol
    Issue Date
    2020-07-16
    Keywords
    PpENVP
    Pteromalus puparum
    Cell Death
    Cytotoxicity
    Encapsulation
    endonuclease
    hemocyte
    Venom
    
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    Publisher
    JOHN WILEY & SONS LTD
    Citation
    Wang, J., Yan, Z., Xiao, S., Wang, B., Fang, Q., Schlenke, T., & Ye, G. (2020). Characterization of a cell death‐inducing endonuclease‐like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Pest Management Science.
    Journal
    PEST MANAGEMENT SCIENCE
    Rights
    Copyright © 2020 Society of Chemical Industry.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    BACKGROUND Parasitoid wasps are valuable natural enemies for controlling pests. To ensure successful parasitism, these wasps inject venoms along with their eggs that are deposited either into or on their hosts. Parasitoid venoms regulate host behaviors, development, metabolism and immune responses. Pteromalus puparum is a pupal endoparasitoid that parasitizes a number of butterflies, including the worldwide pest cabbage butterfly, Pieris rapae. Venom from P. puparum has a variety of effects on host hemocytes, including alteration of absolute and relative hemocyte counts, and inhibition of hemocyte spreading and encapsulation. In particular, P. puparum venom causes hemocyte cell death in vivo and in vitro. RESULTS Using assay-guided chromatography, a cell death-inducing venom fraction was identified and defined as P. puparum endonuclease-like venom protein (PpENVP). It belongs to the DNA/RNA nonspecific endonuclease family, which contains two conserved endonuclease activation sites. We analyzed its expression profiles and demonstrated that PpENVP inhibits gene expression in transfected cells relying on two activation sites. However, RNA interference of PpENVP did not significantly reduce P. puparum venom cytotoxicity, suggesting that PpENVP may not be the sole cytotoxic factor present. CONCLUSION Our results provide novel insight into the function of the P. puparum venom cocktail and identify a promising insecticide candidate endonuclease that targets insect hemocytes.
    Note
    12 month embargo; first published 16 July 2020
    ISSN
    1526-498X
    EISSN
    1526-4998
    PubMed ID
    32673424
    DOI
    10.1002/ps.6011
    Version
    Final accepted manuscript
    ae974a485f413a2113503eed53cd6c53
    10.1002/ps.6011
    Scopus Count
    Collections
    UA Faculty Publications

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