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dc.contributor.authorFritz, Megan L
dc.contributor.authorNunziata, Schyler O
dc.contributor.authorGuo, Rong
dc.contributor.authorTabashnik, Bruce E
dc.contributor.authorCarrière, Yves
dc.date.accessioned2020-11-18T22:30:41Z
dc.date.available2020-11-18T22:30:41Z
dc.date.issued2020-05-01
dc.identifier.citationFritz, M. L., Nunziata, S. O., Guo, R., Tabashnik, B. E., & Carrière, Y. (2020). Mutations in a novel cadherin gene associated with Bt resistance in Helicoverpa zea. G3: Genes, Genomes, Genetics, 10(5), 1563-1574.en_US
dc.identifier.issn2160-1836
dc.identifier.pmid32179620
dc.identifier.doi10.1534/g3.120.401053
dc.identifier.urihttp://hdl.handle.net/10150/648545
dc.description.abstractTransgenic corn and cotton produce crystalline (Cry) proteins derived from the soil bacterium Bacillus thuringiensis (Bt) that are toxic to lepidopteran larvae. Helicoverpa zea, a key pest of corn and cotton in the U.S., has evolved widespread resistance to these proteins produced in Bt corn and cotton. While the genomic targets of Cry selection and the mutations that produce resistant phenotypes are known in other lepidopteran species, little is known about how selection by Cry proteins shape the genome of H. zea We scanned the genomes of Cry1Ac-selected and unselected H. zea lines, and identified twelve genes on five scaffolds that differed between lines, including cadherin-86C (cad-86C), a gene from a family that is involved in Cry1A resistance in other lepidopterans. Although this gene was expressed in the H. zea larval midgut, the protein it encodes has only 17 to 22% identity with cadherin proteins from other species previously reported to be involved in Bt resistance. An analysis of midgut-expressed cDNAs showed significant between-line differences in the frequencies of putative nonsynonymous substitutions (both SNPs and indels). Our results indicate that cad-86C is a likely target of Cry1Ac selection in H. zea It remains unclear, however, whether genomic changes at this locus directly disrupt midgut binding of Cry1Ac and cause Bt resistance, or indirectly enhance fitness of H. zea in the presence of Cry1Ac by some other mechanism. Future work should investigate phenotypic effects of these nonsynonymous substitutions and their impact on fitness of H. zea larvae that ingest Cry1Ac.en_US
dc.language.isoenen_US
dc.publisherGENETICS SOCIETY AMERICAen_US
dc.rightsCopyright © 2020 Fritz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectcadherinen_US
dc.subjectCry1Acen_US
dc.subjectgenome scanningen_US
dc.subjectResistanceen_US
dc.subjectSelectionen_US
dc.titleMutations in a Novel Cadherin Gene Associated with Bt Resistance in Helicoverpa zeaen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Entomolen_US
dc.identifier.journalG3-GENES GENOMES GENETICSen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleG3 (Bethesda, Md.)
dc.source.volume10
dc.source.issue5
dc.source.beginpage1563
dc.source.endpage1574
refterms.dateFOA2020-11-18T22:30:58Z
dc.source.countryUnited States


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Copyright © 2020 Fritz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2020 Fritz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).