Evolutionary and biochemical analyses reveal conservation of the Brassicaceae telomerase ribonucleoprotein complex
AffiliationUniv Arizona, Sch Plant Sci
MetadataShow full item record
PublisherPUBLIC LIBRARY SCIENCE
CitationDew-Budd, K., Cheung, J., Palos, K., Forsythe, E. S., & Beilstein, M. A. (2020). Evolutionary and biochemical analyses reveal conservation of the Brassicaceae telomerase ribonucleoprotein complex. PloS one, 15(4), e0222687.
Rights© 2020 Dew-Budd et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThe telomerase ribonucleoprotein complex (RNP) is essential for genome stability and performs this role through the addition of repetitive DNA to the ends of chromosomes. The telomerase enzyme is composed of a reverse transcriptase (TERT), which utilizes a template domain in an RNA subunit (TER) to reiteratively add telomeric DNA at the ends of chromosomes. Multiple TERs have been identified in the model plant Arabidopsis thaliana. Here we combine a phylogenetic and biochemical approach to understand how the telomerase RNP has evolved in Brassicaceae, the family that includes A. thaliana. Because of the complex phylogenetic pattern of template domain loss and alteration at the previously characterized A. thaliana TER loci, TER1 and TER2, across the plant family Brassicaceae, we bred double mutants from plants with a template deletion at AtTER1 and T-DNA insertion at AtTER2. These double mutants exhibited no telomere length deficiency, a definitive indication that neither of these loci encode a functional telomerase RNA. Moreover, we determined that the telomerase components TERT, Dyskerin, and the KU heterodimer are under strong purifying selection, consistent with the idea that the TER with which they interact is also conserved. To test this hypothesis further, we analyzed the substrate specificity of telomerase from species across Brassicaceae and determined that telomerase from close relatives bind and extend substrates in a similar manner, supporting the idea that TERs in different species are highly similar to one another and are likely encoded from an orthologous locus. Lastly, TERT proteins from across Brassicaceae were able to complement loss of function tert mutants in vivo, indicating TERTs from other species have the ability to recognize the native TER of A. thaliana. Finally, we immunoprecipitated the telomerase complex and identified associated RNAs via RNA-seq. Using our evolutionary data we constrained our analyses to conserved RNAs within Brassicaceae that contained a template domain. These analyses revealed a highly expressed locus whose disruption by a T-DNA resulted in a telomeric phenotype similar to the loss of other telomerase core proteins, indicating that the RNA has an important function in telomere maintenance.
NoteOpen access journal
VersionFinal published version
Except where otherwise noted, this item's license is described as © 2020 Dew-Budd et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
- Evolution of the Arabidopsis telomerase RNA.
- Authors: Beilstein MA, Brinegar AE, Shippen DE
- Issue date: 2012
- An alternative telomerase RNA in Arabidopsis modulates enzyme activity in response to DNA damage.
- Authors: Cifuentes-Rojas C, Nelson AD, Boltz KA, Kannan K, She X, Shippen DE
- Issue date: 2012 Nov 15
- Two RNA subunits and POT1a are components of Arabidopsis telomerase.
- Authors: Cifuentes-Rojas C, Kannan K, Tseng L, Shippen DE
- Issue date: 2011 Jan 4
- Evolution of TERT-interacting lncRNAs: expanding the regulatory landscape of telomerase.
- Authors: Nelson AD, Shippen DE
- Issue date: 2015
- The conserved structure of plant telomerase RNA provides the missing link for an evolutionary pathway from ciliates to humans.
- Authors: Song J, Logeswaran D, Castillo-González C, Li Y, Bose S, Aklilu BB, Ma Z, Polkhovskiy A, Chen JJ, Shippen DE
- Issue date: 2019 Dec 3