Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin
Author
Cha, Byung‐HyunKim, Jin‐Su
Bello, Alvin
Lee, Geun‐Hui
Kim, Do‐Hyun
Kim, Byoung Ju
Arai, Yoshie
Choi, Bogyu
Park, Hansoo
Lee, Soo‐Hong
Affiliation
Univ Arizona, Coll Med, Dept Surg, Div Cardio Thorac SurgIssue Date
2020-07-19
Metadata
Show full item recordPublisher
WILEYCitation
Cha, B.-H., Kim, J.-S., Bello, A., Lee, G.-H., Kim, D.-H., Kim, B. J., ... & Lee, S.-H. (2020). Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin. Advanced Science, 7(17), 2001365.Journal
ADVANCED SCIENCERights
© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation potential of MSCs can be offered the potential to improve the regenerative activity of MSCs therapy. In addition, the underlying processes in the isolation and derivation of MSCs from hPSCs are still poorly understood and controlled. To overcome these clinical needs, an efficient and simplified technique on the isolation of MSCs from spontaneously differentiated human embryonic stem cells (hESCs) via integrin alpha 5 beta 1 (fibronectin (FN) receptor)-to-FN interactions (hESC-FN-MSCs) is successfully developed. It is demonstrated that hESC-FN-MSCs exhibit a typical MSC surface phenotype, cellular morphology, with the whole transcriptome similar to conventional adult MSCs; but show higher proliferative capacity, more efficient trilineage differentiation, enhanced cytokine secretion, and attenuated cellular senescence. In addition, the therapeutic potential and regenerative capacity of the isolated hESC-FN-MSCs are confirmed by in vitro and in vivo multilineage differentiation. This novel method will be useful in the generation of abundant amounts of clinically relevant MSCs for stem cell therapeutics and regenerative medicine.Note
Open access journalISSN
2198-3844EISSN
2198-3844Version
Final published versionae974a485f413a2113503eed53cd6c53
10.1002/advs.202001365
Scopus Count
Collections
Except where otherwise noted, this item's license is described as © 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.