AffiliationUniv Arizona, Canc Biol Grad Interdisciplinary Program
Univ Arizona, Dept Physiol
Univ Arizona, Dept Immunobiol
Univ Arizona, BIO5 Inst
Univ Arizona, Dept Mol & Cellular Biol
MetadataShow full item record
PublisherLIFE SCIENCE ALLIANCE LLC
CitationUhlorn, B. L., Gamez, E. R., Li, S., & Campos, S. K. (2020). Attenuation of cGAS/STING activity during mitosis. Life Science Alliance, 3(9).
JournalLIFE SCIENCE ALLIANCE
Rights© 2020 Uhlorn et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractThe innate immune system recognizes cytosolic DNA associated with microbial infections and cellular stress via the cGAS/STING pathway, leading to activation of phospho-IRF3 and downstream IFN-I and senescence responses. To prevent hyperactivation, cGAS/ STING is presumed to be nonresponsive to chromosomal self-DNA during open mitosis, although specific regulatory mechanisms are lacking. Given a role for the Golgi in STING activation, we investigated the state of the cGAS/STING pathway in interphase cells with artificially vesiculated Golgi and in cells arrested in mitosis. We find that whereas cGAS activity is impaired through interaction with mitotic chromosomes, Golgi integrity has little effect on the enzyme's production of cGAMP. In contrast, STING activation in response to either foreign DNA (cGAS-dependent) or exogenous cGAMP is impaired by a vesiculated Golgi. Overall, our data suggest a secondary means for cells to limit potentially harmful cGAS/ STING responses during open mitosis via natural Golgi vesiculation.
NoteOpen access journal
VersionFinal published version
Except where otherwise noted, this item's license is described as © 2020 Uhlorn et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
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