Cadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollworm
Tabashnik, Bruce E
AffiliationUniv Arizona, Dept Entomol
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CitationWang, L., Ma, Y., Wei, W., Wan, P., Liu, K., Xu, M., ... & Wu, K. (2020). Cadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollworm. Scientific reports, 10(1), 1-10.
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AbstractEvolution of resistance by pests reduces the benefits of transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Here we analyzed resistance to Bt toxin Cry1Ac in a field-derived strain of pink bollworm (Pectinophora gossypiella), a global pest of cotton. We discovered that the r14 allele of the pink bollworm cadherin gene (PgCad1) has a 234-bp insertion in exon 12 encoding a mutant PgCad1 protein that lacks 36 amino acids in cadherin repeat 5 (CR5). A strain homozygous for this allele had 237-fold resistance to Cry1Ac, 1.8-fold cross-resistance to Cry2Ab, and developed from neonate to adult on Bt cotton producing Cry1Ac. Inheritance of resistance to Cry1Ac was recessive and tightly linked with r14. PgCad1 transcript abundance in midgut tissues did not differ between resistant and susceptible larvae. Toxicity of Cry1Ac to transformed insect cells was lower for cells expressing r14 than for cells expressing wild-type PgCad1. Wild-type PgCad1 was transported to the cell membrane, whereas PgCad1 produced by r14 was not. In larval midgut tissue, PgCad1 protein occurred primarily on the brush border membrane only in susceptible larvae. The results imply r14 mediates pink bollworm resistance to Cry1Ac by reduced translation, increased degradation, and/or mislocalization of cadherin.
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Except where otherwise noted, this item's license is described as © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.
- Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20.
- Authors: Wang L, Ma Y, Guo X, Wan P, Liu K, Cong S, Wang J, Xu D, Xiao Y, Li X, Tabashnik BE, Wu K
- Issue date: 2019 Mar 28
- Resistance to Bacillus thuringiensis linked with a cadherin transmembrane mutation affecting cellular trafficking in pink bollworm from China.
- Authors: Wang L, Ma Y, Wan P, Liu K, Xiao Y, Wang J, Cong S, Xu D, Wu K, Fabrick JA, Li X, Tabashnik BE
- Issue date: 2018 Mar
- Similar genetic basis of resistance to Bt toxin Cry1Ac in Boll-selected and diet-selected strains of pink bollworm.
- Authors: Fabrick JA, Tabashnik BE
- Issue date: 2012
- A long non-coding RNA regulates cadherin transcription and susceptibility to Bt toxin Cry1Ac in pink bollworm, Pectinophora gossypiella.
- Authors: Li S, Hussain F, Unnithan GC, Dong S, UlAbdin Z, Gu S, Mathew LG, Fabrick JA, Ni X, Carrière Y, Tabashnik BE, Li X
- Issue date: 2019 Jul
- Reduced cadherin expression associated with resistance to Bt toxin Cry1Ac in pink bollworm.
- Authors: Fabrick JA, Mathew LG, LeRoy DM, Hull JJ, Unnithan GC, Yelich AJ, Carrière Y, Li X, Tabashnik BE
- Issue date: 2020 Jan