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dc.contributor.authorWang, Ling
dc.contributor.authorMa, Yuemin
dc.contributor.authorWei, Wei
dc.contributor.authorWan, Peng
dc.contributor.authorLiu, Kaiyu
dc.contributor.authorXu, Min
dc.contributor.authorCong, Shengbo
dc.contributor.authorWang, Jintao
dc.contributor.authorXu, Dong
dc.contributor.authorXiao, Yutao
dc.contributor.authorLi, Xianchun
dc.contributor.authorTabashnik, Bruce E
dc.contributor.authorWu, Kongming
dc.date.accessioned2021-01-15T21:25:34Z
dc.date.available2021-01-15T21:25:34Z
dc.date.issued2020-10-08
dc.identifier.citationWang, L., Ma, Y., Wei, W., Wan, P., Liu, K., Xu, M., ... & Wu, K. (2020). Cadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollworm. Scientific reports, 10(1), 1-10.en_US
dc.identifier.issn2045-2322
dc.identifier.pmid33033325
dc.identifier.doi10.1038/s41598-020-74102-z
dc.identifier.urihttp://hdl.handle.net/10150/650885
dc.description.abstractEvolution of resistance by pests reduces the benefits of transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Here we analyzed resistance to Bt toxin Cry1Ac in a field-derived strain of pink bollworm (Pectinophora gossypiella), a global pest of cotton. We discovered that the r14 allele of the pink bollworm cadherin gene (PgCad1) has a 234-bp insertion in exon 12 encoding a mutant PgCad1 protein that lacks 36 amino acids in cadherin repeat 5 (CR5). A strain homozygous for this allele had 237-fold resistance to Cry1Ac, 1.8-fold cross-resistance to Cry2Ab, and developed from neonate to adult on Bt cotton producing Cry1Ac. Inheritance of resistance to Cry1Ac was recessive and tightly linked with r14. PgCad1 transcript abundance in midgut tissues did not differ between resistant and susceptible larvae. Toxicity of Cry1Ac to transformed insect cells was lower for cells expressing r14 than for cells expressing wild-type PgCad1. Wild-type PgCad1 was transported to the cell membrane, whereas PgCad1 produced by r14 was not. In larval midgut tissue, PgCad1 protein occurred primarily on the brush border membrane only in susceptible larvae. The results imply r14 mediates pink bollworm resistance to Cry1Ac by reduced translation, increased degradation, and/or mislocalization of cadherin.en_US
dc.language.isoenen_US
dc.publisherNATURE RESEARCHen_US
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleCadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollwormen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Entomolen_US
dc.identifier.journalSCIENTIFIC REPORTSen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleScientific reports
dc.source.volume10
dc.source.issue1
dc.source.beginpage16840
dc.source.endpage
refterms.dateFOA2021-01-15T21:25:35Z
dc.source.countryEngland


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© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.