Preparative High-Performance Liquid Chromatographic Separation of Individual Amino Acids
CitationVan Klinken, G. J., & Mook, W. G. (1990). Preparative high-performance liquid chromatographic separation of individual amino acids derived from fossil bone collagen. Radiocarbon, 32(2), 155-164.
AbstractExtracting a series of amino acids from fossil "collagen" makes it possible to compare chemically well-characterized molecules from one bone sample, both chemically and by isotopic analysis. Thus, the integrity of collagen samples and possible contamination can be checked. We describe the routine high-performance liquid chromatographic extraction of five amino acids: glycine, hydroxyproline, lutamate, threonine and alanine. Humates are eliminated during the extraction, which can be monitored continuously.