Radiocarbon Dating of Bone Osteocalcin: Isolating and Characterizing a Non-Collagen Protein
Citation
Ajie, H. O., Kaplan, I. R., Hauschka, P. V., Kirner, D., Slota, P. J., & Taylor, R. E. (1992). Radiocarbon dating of bone osteocalcin: Isolating and characterizing a non-collagen protein. Radiocarbon, 34(3), 296-305.Journal
RadiocarbonDescription
From the 14th International Radiocarbon Conference held in Tucson, AZ, May 20-24, 1991.Additional Links
http://radiocarbon.webhost.uits.arizona.edu/Abstract
Osteocalcin, a non-collagen bone-matrix protein, has been examined as a possible source of autochthonous 14C data in fossil bones where collagen has been seriously degraded. Extraction procedures for osteocalcin yield a wellcharacterized product that can be clearly distinguished from collagen. The Gla content indicates that osteocalcin is present in the fossil bones at levels similar to the range present in modern bone. However, it appears to be extracted primarily as proteolytic polypeptide fragments rather than as an intact protein. Concordant 14C determinations are obtained on osteocalcin and gelatin extracts from the same bone when the collagen is relatively well preserved. However, increasing discordances in the 14C values of the osteocalcin and gelatin fractions are associated with reduced concentrations of the gelatin extract in the bone.Type
Proceedingstext
Language
enISSN
0033-8222ae974a485f413a2113503eed53cd6c53
10.1017/S0033822200063451