N-6-methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis
Author
Kramer, Marianne C.Janssen, Kevin A.
Palos, Kyle
Nelson, Andrew D. L.
Vandivier, Lee E.
Garcia, Benjamin A.
Lyons, Eric
Beilstein, Mark A.
Gregory, Brian D.
Affiliation
Univ Arizona, Sch Plant SciUniv Arizona, CyVerse
Issue Date
2020-07Keywords
non-coding RNAspost-transcriptional regulation
RNA covalent modifications
RNA processing
RNA stability
RNA-binding proteins
Metadata
Show full item recordPublisher
JOHN WILEY & SONS LTDCitation
Kramer, M. C., Janssen, K. A., Palos, K., Nelson, A. D., Vandivier, L. E., Garcia, B. A., ... & Gregory, B. D. (2020). N6‐methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis. Plant Direct. 2020;00:1–22.Journal
PLANT DIRECTRights
© The Authors. This is an open access article under the terms of the Creative Commons Attribution License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
After transcription, a messenger RNA (mRNA) is further post-transcriptionally regulated by several features including RNA secondary structure and covalent RNA modifications (specifically N-6-methyladenosine, m(6)A). Both RNA secondary structure and m(6)A have been demonstrated to regulate mRNA stability and translation and have been independently linked to plant responses to soil salinity levels. However, the effect of m(6)A on regulating RNA secondary structure and the combinatorial interplay between these two RNA features during salt stress response has yet to be studied. Here, we globally identify RNA-protein interactions and RNA secondary structure during systemic salt stress. This analysis reveals that RNA secondary structure changes significantly during salt stress, and that it is independent of global changes in RNA-protein interactions. Conversely, we find that m(6)A is anti-correlated with RNA secondary structure in a condition-dependent manner, with salt-specific m(6)A correlated with a decrease in mRNA secondary structure during salt stress. Taken together, we suggest that salt-specific m(6)A deposition and the associated loss of RNA secondary structure results in increases in mRNA stability for transcripts encoding abiotic stress response proteins and ultimately increases in protein levels from these stabilized transcripts. In total, our comprehensive analyses reveal important post-transcriptional regulatory mechanisms involved in plant long-term salt stress response and adaptation.Note
Open access journalISSN
2475-4455EISSN
2475-4455PubMed ID
32724893DOI
10.1002/pld3.239Version
Final published versionae974a485f413a2113503eed53cd6c53
10.1002/pld3.239
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Except where otherwise noted, this item's license is described as © The Authors. This is an open access article under the terms of the Creative Commons Attribution License.