Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models
AffiliationUniv Arizona, Coll Med Phoenix, Ctr Appl NanoBiosci & Med
Univ Arizona, Coll Engn, Dept Biomed Engn
MetadataShow full item record
PublisherFRONTIERS MEDIA SA
CitationLacombe, J., Harris, A. F., Zenhausern, R., Karsunsky, S., & Zenhausern, F. (2020). Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models. Frontiers in bioengineering and biotechnology, 8, 932.
RightsCopyright © 2020 Lacombe, Harris, Zenhausern, Karsunsky and Zenhausern. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractPlant-based scaffolds present many advantages over a variety of biomaterials. Recent studies explored their potential to be repopulated with human cells and thus highlight a growing interest for their use in tissue engineering or for biomedical applications. However, it is still unclear if thesein vitroplant-based scaffolds can modify cell phenotype or affect cellular response to external stimuli. Here, we report the characterization of the mechano-regulation of melanoma SK-MEL-28 and prostate PC3 cells seeded on decellularized spinach leaves scaffolds, compared to cells deposited on standard rigid cell culture substrate, as well as their response to drug and radiation treatment. The results showed that YAP/TAZ signaling was downregulated, cellular morphology altered and proliferation rate decreased when cells were cultured on leaf scaffold. Interestingly, cell culture on vegetal scaffold also affected cellular response to external stress. Thus, SK-MEL-28 cells phenotype is modified leading to a decrease in MITF activity and drug resistance, while PC3 cells showed altered gene expression and radiation response. These findings shed lights on the decellularization of vegetal materials to provide substrates that can be repopulated with human cells to better reproduce a soft tissue microenvironment. However, these complex scaffolds mediate changes in cell behavior and in order to exploit the capability of matching physical properties of the various plant scaffolds to diverse physiological functionalities of cells and human tissue constructs, additional studies are required to better characterize physical and biochemical cell-substrate interactions.
NoteOpen access journal
VersionFinal published version
Except where otherwise noted, this item's license is described as Copyright © 2020 Lacombe, Harris, Zenhausern, Karsunsky and Zenhausern. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
- Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds.
- Authors: Harris AF, Lacombe J, Liyanage S, Han MY, Wallace E, Karsunky S, Abidi N, Zenhausern F
- Issue date: 2021 Feb 11
- Substrate stiffness- and topography-dependent differentiation of annulus fibrosus-derived stem cells is regulated by Yes-associated protein.
- Authors: Chu G, Yuan Z, Zhu C, Zhou P, Wang H, Zhang W, Cai Y, Zhu X, Yang H, Li B
- Issue date: 2019 Jul 1
- Efficient mineralization and osteogenic gene overexpression of mesenchymal stem cells on decellularized spinach leaf scaffold.
- Authors: Salehi A, Mobarhan MA, Mohammadi J, Shahsavarani H, Shokrgozar MA, Alipour A
- Issue date: 2020 Oct 5
- Bioreactivity of decellularized animal, plant, and fungal scaffolds: perspectives for medical applications.
- Authors: Predeina AL, Dukhinova MS, Vinogradov VV
- Issue date: 2020 Nov 18
- Decellularized cartilage matrix scaffolds with laser-machined micropores for cartilage regeneration and articular cartilage repair.
- Authors: Li Y, Xu Y, Liu Y, Wang Z, Chen W, Duan L, Gu D
- Issue date: 2019 Dec