A low-cost computational approach to analyze spiking activity in cockroach sensory neurons
AffiliationDepartment of Neuroscience, The University of Arizona
MetadataShow full item record
PublisherAmerican Physiological Society
CitationTorres, D. J., Romero, A., Colgan III, W., & Ricoy, U. M. (2021). A low-cost computational approach to analyze spiking activity in cockroach sensory neurons. Advances in Physiology Education, 45(1), 145-153.
JournalAdvances in Physiology Education
RightsCopyright © 2021 the American Physiological Society
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractUndergraduates use a spike sorting routine developed in Octave to analyze the spiking activity generated from mechanical stimulation of spines of cockroach legs with the inexpensive SpikerBox amplifier and the free software Audacity. Students learn the procedures involved in handling the cockroaches and recording extracellular action potentials (spikes) with the SpikerBox apparatus as well as the importance of spike sorting for analysis in neuroscience. The spike sorting process requires students to choose the spike threshold and spike selection criteria and interact with the clustering process that forms the groups of similar spikes. Once the spike groups are identified, interspike intervals and neuron firing frequencies can be calculated and analyzed. A classic neurophysiology lab exercise is thus adapted to be interdisciplinary for underrepresented students in a small rural college. © 2021 the American Physiological Society. All Rights Reserved
Note12 month embargo; first published online 4 March 2021
VersionFinal accepted manuscript
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