Show simple item record

dc.contributor.authorValdivia‐Carrillo, Tania
dc.contributor.authorRocha‐Olivares, Axayácatl
dc.contributor.authorReyes‐Bonilla, Héctor
dc.contributor.authorDomínguez‐Contreras, José Francisco
dc.contributor.authorMunguía-Vega, Adrián
dc.date.accessioned2021-05-05T19:59:00Z
dc.date.available2021-05-05T19:59:00Z
dc.date.issued2021-03-08
dc.identifier.citationValdivia‐Carrillo, T., Rocha‐Olivares, A., Reyes‐Bonilla, H., Domínguez‐Contreras, J. F., & Munguia‐Vega, A. (2021). Integrating eDNA metabarcoding and simultaneous underwater visual surveys to describe complex fish communities in a marine biodiversity hotspot. Molecular Ecology Resources.en_US
dc.identifier.issn1755-098X
dc.identifier.doi10.1111/1755-0998.13375
dc.identifier.urihttp://hdl.handle.net/10150/658167
dc.description.abstractMarine biodiversity can be surveyed using underwater visual censuses and recently with eDNA metabarcoding. Although a promising tool, eDNA studies have shown contrasting results related to its detection scale and the number of species identified compared to other survey methods. Also, its accuracy relies on complete reference databases used for taxonomic assignment and, as other survey methods, species detection may show false-negative and false-positive errors. Here, we compared results from underwater visual censuses and simultaneous eDNA metabarcoding fish surveys in terms of observed species and community composition. We also assess the effect of a custom reference database in the taxonomic assignment, and evaluate occupancy, capture and detection probabilities, as well as error rates of eDNA survey data. We amplified a 12S rRNA fish barcode from 24 sampling sites in the gulf of California. More species were detected with eDNA metabarcoding than with UVC. Because each survey method largely detected different sets of species, the combined approach doubled the number of species registered. Both survey methods recovered a known biodiversity gradient and a biogeographic break, but eDNA captured diversity over a broader geographic and bathymetric scale. Furthermore, the use of a modest-sized custom reference database significantly increased taxonomic assignment. In a subset of species, occupancy models revealed eDNA surveys provided similar or higher detection probabilities compared to UVC. The occupancy value of each species had a large influence on eDNA detectability, and in the false positive and negative error. Overall, these results highlight the potential of eDNA metabarcoding in complementing other established ecological methods for studies of marine fishes. © 2021 John Wiley & Sons Ltden_US
dc.description.sponsorshipDavid and Lucile Packard Foundationen_US
dc.language.isoenen_US
dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2021 John Wiley & Sons Ltd.en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.subjectbiodiversity monitoringen_US
dc.subjectdetection probabilityen_US
dc.subjecteDNA metabarcodingen_US
dc.subjectfish communityen_US
dc.subjectoccupancy modelen_US
dc.subjectreference databaseen_US
dc.subjectunderwater visual censusen_US
dc.titleIntegrating eDNA metabarcoding and simultaneous underwater visual surveys to describe complex fish communities in a marine biodiversity hotspoten_US
dc.typeArticleen_US
dc.identifier.eissn1755-0998
dc.contributor.departmentConservation Genetics Laboratory & Desert Laboratory on Tumamoc Hill, The University of Arizonaen_US
dc.identifier.journalMolecular Ecology Resourcesen_US
dc.description.note12 month embargo; first published online 8 March 2021en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleMolecular Ecology Resources


Files in this item

Thumbnail
Name:
MER-20-0450.R1_Proof_hi.pdf
Size:
1.932Mb
Format:
PDF
Description:
Final Accepted Manuscript

This item appears in the following Collection(s)

Show simple item record