Detection of viral RNA fragments in human iPSC cardiomyocytes following treatment with extracellular vesicles from SARS-CoV-2 coding sequence overexpressing lung epithelial cells
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Kwon, YoujeongNukala, Sarath Babu
Srivastava, Shubhi
Miyamoto, Hiroe
Ismail, Nur Izzah
Jousma, Jordan
Rehman, Jalees
Ong, Sang-Bing
Lee, Won Hee
Ong, Sang-Ging
Affiliation
Univ Arizona, Dept Basic Med Sci, Coll Med PhoenixIssue Date
2020-11-30
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Kwon, Y., Nukala, S. B., Srivastava, S., Miyamoto, H., Ismail, N. I., Jousma, J., ... & Ong, S. G. (2020). Detection of viral RNA fragments in human iPSC cardiomyocytes following treatment with extracellular vesicles from SARS-CoV-2 coding sequence overexpressing lung epithelial cells. Stem Cell Research & Therapy, 11(1), 1-5.Journal
STEM CELL RESEARCH & THERAPYRights
© The Author(s). 2020 Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic. The prevalence/severity of COVID-19 is higher among patients with cardiovascular risk factors. Despite the expression of angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2 infection, in cardiomyocytes, there has been no conclusive evidence of direct viral infection although the presence of viral genome within COVID-19 patients' hearts has been reported. Here, we overexpressed SARS-CoV-2 genes in A549 lung epithelial cells. We then isolated extracellular vesicles (EVs) and detected the presence of viral RNA within these EVs. We observed that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are receptive to these EVs, and viral genes were detectable in the cardiomyocytes. Accordingly, the uptake of viral RNA-harboring EVs led to an upregulation of inflammation-related genes in hiPSC-CMs. Thus, our findings indicate that SARS-CoV-2 RNA containing EVs represents an indirect route of viral RNA entry into cardiomyocytes.Note
Open access articleISSN
1757-6512EISSN
1757-6512PubMed ID
33256833Version
Final published versionae974a485f413a2113503eed53cd6c53
10.1186/s13287-020-02033-7
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Except where otherwise noted, this item's license is described as © The Author(s). 2020 Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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