AdvisorRogers, Gregory C.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
EmbargoRelease after 05/14/2022
AbstractThe centrosome is a tiny organelle that builds and coordinates complex microtubule machines in cells. Centrioles, the core components of each centrosome, are barrel-shaped structures with intricate architecture. Establishing and maintaining proper centriole structure is critical for proper centrosome function; however, deciphering mechanisms underlying centriole assembly remains a major hurdle in the field of centrosome biology. To address this gap, I sought to elucidate mechanisms by which the centriole grows. Herein, I focus on a key question in the field: How do the proteins that reside at the distal tip of the centriole coordinate processive growth during specific stages of centriole biogenesis? First, I identify a novel component of the conserved distal tip complex, Cep104, that promotes centriole growth. Second, I decipher a mechanism regulating activity of the key centriolar kinase Polo-like Kinase 4 and provide a potential role for the kinase in regulating the distal tip complex. By combining these findings with a detailed distal tip interactome, I develop a hypothetical model of centriole assembly integrating our genetic understanding of centriole growth with my novel molecular mechanisms.
Degree ProgramGraduate College
Cellular & Molecular Medicine