Show simple item record

dc.contributor.advisorRogers, Gregory C.
dc.contributor.authorRyniawec, John
dc.creatorRyniawec, John
dc.date.accessioned2021-05-18T21:10:05Z
dc.date.available2021-05-18T21:10:05Z
dc.date.issued2021
dc.identifier.citationRyniawec, John. (2021). Regulation of Centriole Length by the Distal Tip Complex (Doctoral dissertation, University of Arizona, Tucson, USA).
dc.identifier.urihttp://hdl.handle.net/10150/658586
dc.description.abstractThe centrosome is a tiny organelle that builds and coordinates complex microtubule machines in cells. Centrioles, the core components of each centrosome, are barrel-shaped structures with intricate architecture. Establishing and maintaining proper centriole structure is critical for proper centrosome function; however, deciphering mechanisms underlying centriole assembly remains a major hurdle in the field of centrosome biology. To address this gap, I sought to elucidate mechanisms by which the centriole grows. Herein, I focus on a key question in the field: How do the proteins that reside at the distal tip of the centriole coordinate processive growth during specific stages of centriole biogenesis? First, I identify a novel component of the conserved distal tip complex, Cep104, that promotes centriole growth. Second, I decipher a mechanism regulating activity of the key centriolar kinase Polo-like Kinase 4 and provide a potential role for the kinase in regulating the distal tip complex. By combining these findings with a detailed distal tip interactome, I develop a hypothetical model of centriole assembly integrating our genetic understanding of centriole growth with my novel molecular mechanisms.
dc.language.isoen
dc.publisherThe University of Arizona.
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
dc.subjectCentriole
dc.subjectCentriole Length
dc.subjectCentrosome
dc.subjectDistal Tip Complex
dc.subjectPolo-like Kinase 4
dc.titleRegulation of Centriole Length by the Distal Tip Complex
dc.typetext
dc.typeElectronic Dissertation
thesis.degree.grantorUniversity of Arizona
thesis.degree.leveldoctoral
dc.contributor.committeememberSchroeder, Joyce
dc.contributor.committeememberMouneimne, Ghassan
dc.contributor.committeememberKrieg, Paul
dc.description.releaseRelease after 05/14/2022
thesis.degree.disciplineGraduate College
thesis.degree.disciplineCellular & Molecular Medicine
thesis.degree.namePh.D.


Files in this item

Thumbnail
Name:
azu_etd_18648_sip1_m.pdf
Size:
5.258Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record