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dc.contributor.authorLago-Alvarez, Y.
dc.contributor.authorPodico, G.
dc.contributor.authorSegabinazzi, L.G.
dc.contributor.authorCunha, L.L.
dc.contributor.authorBarbosa, L.
dc.contributor.authorArnold, C.E.
dc.contributor.authorLima, F.S.
dc.contributor.authorKing, L.T.
dc.contributor.authorMcLean, A.K.
dc.contributor.authorCanisso, I.F.
dc.date.accessioned2021-06-04T02:36:34Z
dc.date.available2021-06-04T02:36:34Z
dc.date.issued2020
dc.identifier.citationLago-Alvarez, Y., Podico, G., Segabinazzi, L. G., Cunha, L. L., Barbosa, L., Arnold, C. E., ... & Canisso, I. F. (2020). Donkey Epididymal Transport for Semen Cooling and Freezing. Animals, 10(12), 2209.
dc.identifier.issn2076-2615
dc.identifier.doi10.3390/ani10122209
dc.identifier.urihttp://hdl.handle.net/10150/659653
dc.description.abstractThe objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, n = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, n = 14) or dissected apart (Experiment 3, n = 36). In each experiment, semen was frozen immediately (Non-Centrif) in an egg yolk-based semen extender (EY) or after processing through cushion-centrifugation (Centrif) while extended in a skim milk-based extender (SC). In all three experiments, cooled, pre-freeze, and post-thaw epididymal semen was assessed for total motility (TM), progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP). Data were analyzed with R using mixed models and Tukey’s test as posthoc. Results showed that the cooling of epididymal semen up to 24 h after harvesting did not affect motility parameters or plasma membrane integrity; furthermore, in Experiment 3, the post-thaw evaluation of both Centrif and Non-Centrif achieved similar TM and PM. Collectively, the post-thaw results revealed low motility parameters across groups; while, the PMI and HMMP did not reflect this trend, and the values remained high, suggesting that there was a lack of epididymal sperm activation with either centrifugation or extenders. In summary, freshly harvested and cooled-shipped and cooled semen had satisfactory semen parameters. Future studies need to address donkey epididymal semen fertility in mares and jennies. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
dc.language.isoen
dc.publisherMDPI AG
dc.rightsCopyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCastration
dc.subjectCooled-shipped epididymides
dc.subjectEpididymis
dc.subjectSemen cryopreservation
dc.subjectTestis
dc.titleDonkey epididymal transport for semen cooling and freezing
dc.typeArticle
dc.typetext
dc.contributor.departmentDepartment of Veterinary Clinical Medicine, University of Arizona
dc.identifier.journalAnimals
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleAnimals
refterms.dateFOA2021-06-04T02:36:34Z


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Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).