Human sensor-inspired supervised machine learning of smartphone-based paper microfluidic analysis for bacterial species classification
Lee, Min Hee
Day, Alexander S
Hwang, Dong Soo
AffiliationDepartment of Biosystems Engineering, The University of Arizona
Department of Biomedical Engineering, The University of Arizona
Paper microfluidic chip
Support vector machine (SVM)
MetadataShow full item record
CitationKim, S., Lee, M. H., Wiwasuku, T., Day, A. S., Youngme, S., Hwang, D. S., & Yoon, J. Y. (2021). Human sensor-inspired supervised machine learning of smartphone-based paper microfluidic analysis for bacterial species classification. Biosensors and Bioelectronics, 113335.
JournalBiosensors & bioelectronics
RightsCopyright © 2021 Elsevier B.V. All rights reserved.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractBacteria identification has predominantly been conducted using specific bioreceptors such as antibodies or nucleic acid sequences. This approach may be inappropriate for environmental monitoring when the user does not know the target bacterial species and for screening complex water samples with many unknown bacterial species. In this work, we investigate the supervised machine learning of the bacteria-particle aggregation pattern induced by the peptide sets identified from the biofilm-bacteria interface. Each peptide is covalently conjugated to polystyrene particles and loaded together with bacterial suspensions onto paper microfluidic chips. Each peptide interacts with bacterial species to a different extent, leading to varying sizes of particle aggregation. This aggregation changes the surface tension and viscosity of the liquid flowing through the paper pores, altering the flow velocity at different extents. A smartphone camera captures this flow velocity without being affected by ambient and environmental conditions, towards a low-cost, rapid, and field-ready assay. A collection of such flow velocity data generates a unique fingerprinting profile for each bacterial species. Support vector machine is utilized to classify the species. At optimized conditions, the training model can predict the species at 93.3% accuracy out of five bacteria: Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium, Enterococcus faecium, and Pseudomonas aeruginosa. Flow rates are monitored for less than 6 s and the sample-to-answer assay time is less than 10 min. The demonstrated method can open a new way of analyzing complex biological and environmental samples in a biomimetic manner with machine learning classification. © 2021 Elsevier B.V.
Note24 month embargo; available online 14 May 2021
VersionFinal accepted manuscript
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