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dc.contributor.authorRyan, Anne R
dc.contributor.authorEngland, Alicia R
dc.contributor.authorChaney, Christopher P
dc.contributor.authorCowdin, Mitzy A
dc.contributor.authorHiltabidle, Max
dc.contributor.authorDaniel, Edward
dc.contributor.authorGupta, Ashwani Kumar
dc.contributor.authorOxburgh, Leif
dc.contributor.authorCarroll, Thomas J
dc.contributor.authorCleaver, Ondine
dc.date.accessioned2021-06-11T03:07:37Z
dc.date.available2021-06-11T03:07:37Z
dc.date.issued2021-05-15
dc.identifier.citationRyan, A. R., England, A. R., Chaney, C. P., Cowdin, M. A., Hiltabidle, M., Daniel, E., Gupta, A. K., Oxburgh, L., Carroll, T. J., & Cleaver, O. (2021). Vascular deficiencies in renal organoids and ex vivo kidney organogenesis. Developmental Biology, 477, 98–116.en_US
dc.identifier.pmid34000274
dc.identifier.doi10.1016/j.ydbio.2021.04.009
dc.identifier.urihttp://hdl.handle.net/10150/659875
dc.description.abstractChronic kidney disease (CKD) and end stage renal disease (ESRD) are increasingly frequent and devastating conditions that have driven a surge in the need for kidney transplantation. A stark shortage of organs has fueled interest in generating viable replacement tissues ex vivo for transplantation. One promising approach has been self-organizing organoids, which mimic developmental processes and yield multicellular, organ-specific tissues. However, a recognized roadblock to this approach is that many organoid cell types fail to acquire full maturity and function. Here, we comprehensively assess the vasculature in two distinct kidney organoid models as well as in explanted embryonic kidneys. Using a variety of methods, we show that while organoids can develop a wide range of kidney cell types, as previously shown, endothelial cells (ECs) initially arise but then rapidly regress over time in culture. Vasculature of cultured embryonic kidneys exhibit similar regression. By contrast, engraftment of kidney organoids under the kidney capsule results in the formation of a stable, perfused vasculature that integrates into the organoid. This work demonstrates that kidney organoids offer a promising model system to define the complexities of vascular-nephron interactions, but the establishment and maintenance of a vascular network present unique challenges when grown ex vivo.en_US
dc.language.isoenen_US
dc.publisherElsevier Inc.en_US
dc.rightsCopyright © 2021. Published by Elsevier Inc.en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.subjectAngioblasten_US
dc.subjectBlood vesselen_US
dc.subjectCorden_US
dc.subjectEndothelial cellen_US
dc.subjectKidneyen_US
dc.subjectOrganoiden_US
dc.subjectRNAseqen_US
dc.titleVascular deficiencies in renal organoids and ex vivo kidney organogenesisen_US
dc.typeArticleen_US
dc.identifier.eissn1095-564X
dc.contributor.departmentUniversity of Arizonaen_US
dc.identifier.journalDevelopmental Biologyen_US
dc.description.note12 month embargo; available online 15 May 2021en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleDevelopmental biology
dc.source.volume477
dc.source.beginpage98
dc.source.endpage116
dc.source.countryUnited States


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