Automated Laboratory Growth Assessment and Maintenance of Azotobacter vinelandii
AffiliationDepartment of Molecular and Cellular Biology, University of Arizona
Department of Astronomy and Steward Observatory, University of Arizona
Lunar and Planetary Laboratory, University of Arizona
MetadataShow full item record
PublisherBlackwell Publishing Inc.
CitationCarruthers, B. M., Garcia, A. K., Rivier, A., & Kacar, B. (2021). Automated Laboratory Growth Assessment and Maintenance of Azotobacter vinelandii. Current Protocols, 1(3), e57.
RightsCopyright © 2021 The Authors. This is an open access article under the terms of the Creative Commons Attribution License.
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AbstractAzotobacter vinelandii (A. vinelandii) is a commonly used model organism for the study of aerobic respiration, the bacterial production of several industrially relevant compounds, and, perhaps most significantly, the genetics and biochemistry of biological nitrogen fixation. Laboratory growth assessments of A. vinelandii are useful for evaluating the impact of environmental and genetic modifications on physiological properties, including diazotrophy. However, researchers typically rely on manual growth methods that are oftentimes laborious and inefficient. We present a protocol for the automated growth assessment of A. vinelandii on a microplate reader, particularly well-suited for studies of diazotrophic growth. We discuss common pitfalls and strategies for protocol optimization, and demonstrate the protocol's application toward growth evaluation of strains carrying modifications to nitrogen-fixation genes. © 2021 The Authors. Basic Protocol 1: Preparation of A. vinelandii plate cultures from frozen stock. Basic Protocol 2: Preparation of A. vinelandii liquid precultures. Basic Protocol 3: Automated growth rate experiment of A. vinelandii on a microplate reader. © 2021 The Authors.
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Except where otherwise noted, this item's license is described as Copyright © 2021 The Authors. This is an open access article under the terms of the Creative Commons Attribution License.