Light Profile Microscopy
Publisher
The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
The performance of many biomedical optical imaging devices, including confocal and light sheet microscopes, depends heavily on the light beam characteristics such as beam width and depth of focus. However, these characteristics can be significantly affected when imaging in deep tissue, as tissue is a highly scattering medium. The current research outlines the design of the Light Profile Microscope, which directly observes the beam width focused inside biological tissue. The device was designed using SOLIDWORKS and assembled with 3D printed parts and off-the-shelf optics. The performance was tested using pork belly and a sheet of translucent plastic.Type
textElectronic Thesis
Degree Name
M.S.Degree Level
mastersDegree Program
Graduate CollegeOptical Sciences