Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
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Collagenkerateinemultiproteinh ...
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College of Biomedical Engineering, The University of ArizonaIssue Date
2021
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Taylor and Francis Ltd.Citation
Zuniga, K., Gadde, M., Scheftel, J., Senecal, K., Cressman, E., Van Dyke, M., & Rylander, M. N. (2021). Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models. International Journal of Hyperthermia, 38(1), 830–845.Rights
Copyright © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Objective: To determine whether the addition of kerateine (reduced keratin) in rat tail collagen type I hydrogels increases thermal stability and changes material properties and supports cell growth for use in cellular hyperthermia studies for tumor treatment. Methods: Collagen type I extracted from rat tail tendon was combined with kerateine extracted from human hair fibers. Thermal, mechanical, and biocompatibility properties and cell behavior was assessed and compared to 100% collagen type I hydrogels to demonstrate their utility as a tissue model for 3D in vitro testing. Results: A combination (i.e., containing both collagen ‘C/KNT’) hydrogel was more thermally stable than pure collagen hydrogels and resisted thermal degradation when incubated at a hyperthermic temperature of 47°C for heating durations up to 60 min with a higher melting temperature measured by DSC. An increase in the storage modulus was only observed with an increased collagen concentration rather than an increased KTN concentration; however, a change in ECM structure was observed with greater fiber alignment and width with an increase in KTN concentration. The C/KTN hydrogels, specifically 50/50 C/KTN hydrogels, also supported the growth and of fibroblasts and MDA-MB-231 breast cancer cells similar to those seeded in 100% collagen hydrogels. Conclusion: This multi-protein C/KTN hydrogel shows promise for future studies involving thermal stress studies without compromising the 3D ECM environment or cell growth. © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.Note
Open access journalISSN
0265-6736PubMed ID
34058945Version
Final published versionae974a485f413a2113503eed53cd6c53
10.1080/02656736.2021.1930202
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Except where otherwise noted, this item's license is described as Copyright © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
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