Analysis of the Coptis chinensis genome reveals the diversification of protoberberine-type alkaloids
AffiliationDepartment of Ecology and Evolutionary Biology, University of Arizona
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CitationLiu, Y., Wang, B., Shu, S., Li, Z., Song, C., Liu, D., Niu, Y., Liu, J., Zhang, J., Liu, H., Hu, Z., Huang, B., Liu, X., Liu, W., Jiang, L., Alami, M. M., Zhou, Y., Ma, Y., He, X., … Nie, J. (2021). Analysis of the Coptis chinensis genome reveals the diversification of protoberberine-type alkaloids. Nature Communications, 12(1).
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AbstractChinese goldthread (Coptis chinensis Franch.), a member of the Ranunculales, represents an important early-diverging eudicot lineage with diverse medicinal applications. Here, we present a high-quality chromosome-scale genome assembly and annotation of C. chinensis. Phylogenetic and comparative genomic analyses reveal the phylogenetic placement of this species and identify a single round of ancient whole-genome duplication (WGD) shared by the Ranunculaceae. We characterize genes involved in the biosynthesis of protoberberine-type alkaloids in C. chinensis. In particular, local genomic tandem duplications contribute to member amplification of a Ranunculales clade-specific gene family of the cytochrome P450 (CYP) 719. The functional versatility of a key CYP719 gene that encodes the (S)-canadine synthase enzyme involved in the berberine biosynthesis pathway may play critical roles in the diversification of other berberine-related alkaloids in C. chinensis. Our study provides insights into the genomic landscape of early-diverging eudicots and provides a valuable model genome for genetic and applied studies of Ranunculales. © 2021, The Author(s).
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Except where otherwise noted, this item's license is described as Copyright © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License.
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