Automated microscope-independent fluorescence-guided micropipette
AffiliationUniversity of Arizona, College of Medicine – Phoenix
MetadataShow full item record
PublisherThe Optical Society
CitationMiranda, C., Howell, M. R., Lusk, J. F., Marschall, E., Eshima, J., Anderson, T., & Smith, B. S. (2021). Automated microscope-independent fluorescence-guided micropipette. Biomedical Optics Express, 12(8).
JournalBiomedical Optics Express
RightsCopyright © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractGlass micropipette electrodes are commonly used to provide high resolution recordings of neurons. Although it is the gold standard for single cell recordings, it is highly dependent on the skill of the electrophysiologist. Here, we demonstrate a method of guiding micropipette electrodes to neurons by collecting fluorescence at the aperture, using an intra-electrode tapered optical fiber. The use of a tapered fiber for excitation and collection of fluorescence at the micropipette tip couples the feedback mechanism directly to the distance between the target and electrode. In this study, intra-electrode tapered optical fibers provide a targeted robotic approach to labeled neurons that is independent of microscopy. © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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