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    Automated microscope-independent fluorescence-guided micropipette

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    boe-12-8-4689.pdf
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    Author
    Miranda, C.
    Howell, M.R.
    Lusk, J.F.
    Marschall, E.
    Eshima, J.
    Anderson, T.
    Smith, B.S.
    Affiliation
    University of Arizona, College of Medicine – Phoenix
    Issue Date
    2021
    
    Metadata
    Show full item record
    Publisher
    The Optical Society
    Citation
    Miranda, C., Howell, M. R., Lusk, J. F., Marschall, E., Eshima, J., Anderson, T., & Smith, B. S. (2021). Automated microscope-independent fluorescence-guided micropipette. Biomedical Optics Express, 12(8).
    Journal
    Biomedical Optics Express
    Rights
    Copyright © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Glass micropipette electrodes are commonly used to provide high resolution recordings of neurons. Although it is the gold standard for single cell recordings, it is highly dependent on the skill of the electrophysiologist. Here, we demonstrate a method of guiding micropipette electrodes to neurons by collecting fluorescence at the aperture, using an intra-electrode tapered optical fiber. The use of a tapered fiber for excitation and collection of fluorescence at the micropipette tip couples the feedback mechanism directly to the distance between the target and electrode. In this study, intra-electrode tapered optical fibers provide a targeted robotic approach to labeled neurons that is independent of microscopy. © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
    Note
    Open access journal
    ISSN
    2156-7085
    DOI
    10.1364/BOE.431372
    Version
    Final published version
    ae974a485f413a2113503eed53cd6c53
    10.1364/BOE.431372
    Scopus Count
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    UA Faculty Publications

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