Shear-Mediated Platelet Activation is Accompanied by Unique Alterations in Platelet Release of Lipids
Wise, Lisa M.
Arce, Fernando Teran
Saavedra, S. Scott
Slepian, Marvin J.
Purdy, John G.
AffiliationDepartment of Biomedical Engineering, University of Arizona
Department of Immunobiology, University of Arizona
BIO5 Institute, University of Arizona
Department of Medicine, Sarver Heart Center, University of Arizona
Department of Chemistry and Biochemistry, University of Arizona
Department of Material Sciences and Engineering, University of Arizona
Mechanical circulatory support
MetadataShow full item record
PublisherSpringer Science and Business Media LLC
CitationSweedo, A., Wise, L. M., Roka-Moiia, Y., Arce, F. T., Saavedra, S. S., Sheriff, J., Bluestein, D., Slepian, M. J., & Purdy, J. G. (2021). Shear-Mediated Platelet Activation is Accompanied by Unique Alterations in Platelet Release of Lipids. Cellular and Molecular Bioengineering.
RightsCopyright © 2021 Biomedical Engineering Society.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractIntroduction: Platelet activation by mechanical means such as shear stress exposure, is a vital driver of thrombotic risk in implantable blood-contacting devices used in the treatment of heart failure. Lipids are essential in platelets activation and have been studied following biochemical activation. However, little is known regarding lipid alterations occurring with mechanical shear-mediated platelet activation. Methods: Here, we determined if shear-activation of platelets induced lipidome changes that differ from those associated with biochemically-mediated platelet activation. We performed high-resolution lipidomic analysis on purified platelets from four healthy human donors. For each donor, we compared the lipidome of platelets that were non-activated or activated by shear, ADP, or thrombin treatment. Results: We found that shear activation altered cell-associated lipids and led to the release of lipids into the extracellular environment. Shear-activated platelets released 21 phospholipids and sphingomyelins at levels statistically higher than platelets activated by biochemical stimulation. Conclusions: We conclude that shear-mediated activation of platelets alters the basal platelet lipidome. Further, these alterations differ and are unique in comparison to the lipidome of biochemically activated platelets. Many of the released phospholipids contained an arachidonic acid tail or were phosphatidylserine lipids, which have known procoagulant properties. Our findings suggest that lipids released by shear-activated platelets may contribute to altered thrombosis in patients with implanted cardiovascular therapeutic devices. © 2021, Biomedical Engineering Society.
Note12 month embargo; published: 25 August 2021
VersionFinal accepted manuscript
SponsorsArizona Biomedical Research Commission