The SEEL motif and members of the MYB-related REVEILLE transcription factor family are important for the expression of LORELEI in the synergid cells of the Arabidopsis female gametophyte
Name:
Noble_etal_2021_Accepted_Manus ...
Size:
657.8Kb
Format:
PDF
Description:
Final Accepted Manuscript
Name:
Noble_etal_Supplementary_Mater ...
Size:
2.210Mb
Format:
PDF
Description:
Supplementary Material
Name:
Noble_etal_Supplementary Datas ...
Size:
4.532Mb
Format:
application/zip
Description:
Supplementary Data
Author
Noble, Jennifer A.Seddon, Alex
Uygun, Sahra
Bright, Ashley
Smith, Steven E.
Shiu, Shin-han
Palanivelu, Ravishankar

Affiliation
School of Plant Sciences, University of ArizonaSchool of Natural Resources and the Environment, University of Arizona
Issue Date
2021-10-29
Metadata
Show full item recordPublisher
Springer Science and Business Media LLCCitation
Noble, J. A., Seddon, A., Uygun, S., Bright, A., Smith, S. E., Shiu, S.-H., & Palanivelu, R. (2021). The SEEL motif and members of the MYB-related REVEILLE transcription factor family are important for the expression of LORELEI in the synergid cells of the Arabidopsis female gametophyte. Plant Reproduction.Journal
Plant ReproductionRights
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Synergid cells in the micropylar end of the female gametophyte are required for critical cell–cell signaling interactions between the pollen tube and the ovule that precede double fertilization and seed formation in flowering plants. LORELEI (LRE) encodes a putative GPI-anchored protein that is expressed primarily in the synergid cells, and together with FERONIA, a receptor-like kinase, it controls pollen tube reception by the receptive synergid cell. Still, how LRE expression is controlled in synergid cells remains poorly characterized. We identified candidate cis-regulatory elements enriched in LRE and other synergid cell-expressed genes. One of the candidate motifs (‘TAATATCT’) in the LRE promoter was an uncharacterized variant of the Evening Element motif that we named as the Short Evening Element-like (SEEL) motif. Deletion or point mutations in the SEEL motif of the LRE promoter resulted in decreased reporter expression in synergid cells, demonstrating that the SEEL motif is important for expression of LRE in synergid cells. Additionally, we found that LRE expression is decreased in the loss of function mutants of REVEILLE (RVE) transcription factors, which are clock genes known to bind the SEEL and other closely related motifs. We propose that RVE transcription factors regulate LRE expression in synergid cells by binding to the SEEL motif in the LRE promoter. Identification of cis-regulatory elements and transcription factors involved in the expression of LRE will serve as a foundation to characterize the gene regulatory networks in synergid cells.Note
12 month embargo; published: 29 October 2021ISSN
2194-7953EISSN
2194-7961Version
Final accepted manuscriptSponsors
National Science Foundationae974a485f413a2113503eed53cd6c53
10.1007/s00497-021-00432-1