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dc.contributor.authorMorrison, C.
dc.contributor.authorWeterings, E.
dc.contributor.authorMahadevan, D.
dc.contributor.authorSanan, A.
dc.contributor.authorWeinand, M.
dc.contributor.authorStea, B.
dc.date.accessioned2021-11-29T20:25:02Z
dc.date.available2021-11-29T20:25:02Z
dc.date.issued2021
dc.identifier.citationMorrison, C., Weterings, E., Mahadevan, D., Sanan, A., Weinand, M., & Stea, B. (2021). Expression levels of rad51 inversely correlate with survival of glioblastoma patients. Cancers.
dc.identifier.issn2072-6694
dc.identifier.doi10.3390/cancers13215358
dc.identifier.urihttp://hdl.handle.net/10150/662404
dc.description.abstractTreatment failures of glioblastoma (GBM) occur within high-dose radiation fields. We hypothesized that this is due to increased capacity for DNA damage repair in GBM. We identified 24 adult GBM patients treated with maximal safe resection followed by radiation with concurrent and adjuvant temozolomide. The mRNA from patients was quantified using NanoString Technologies’ nCounter platform and compared with 12 non-neoplastic temporal lobe tissue samples as a control. Differential expression analysis identified seven DNA repair genes significantly upregulated in GBM tissues relative to controls (>4-fold difference, adjusted p values < 0.001). Among these seven genes, Cox proportional hazards models identified RAD51 to be associated with an increased risk of death (HR = 3.49; p = 0.03). Kaplan–Meier (KM) analysis showed that patients with high RAD51 expression had significantly shorter OS compared to low levels (median OS of 10.6 mo. vs 20.1 mo.; log-rank p = 0.03). Our findings were validated in a larger external dataset of 162 patients using publicly available gene expression data quantified by the same NanoString technology (median OS of 13.8 mo. vs. 17.4 mo; log-rank p = 0.006). Within this uniformly treated GBM population, RAD51, in the homologous recombination pathway, was overexpressed (vs. normal brain) and inversely correlated with OS. High RAD51 expression may be a prognostic biomarker and a therapeutic target in GBM. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.language.isoen
dc.publisherMDPI
dc.rightsCopyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGBM
dc.subjectGene expression
dc.subjectNanoString nCounter
dc.subjectPrognostic marker
dc.subjectRAD51
dc.titleExpression levels of rad51 inversely correlate with survival of glioblastoma patients
dc.typeArticle
dc.typetext
dc.contributor.departmentDepartment of Radiation Oncology, University of Arizona
dc.contributor.departmentDepartment of Neurosurgery, University of Arizona College of Medicine
dc.identifier.journalCancers
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleCancers
refterms.dateFOA2021-11-29T20:25:02Z


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Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).