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dc.contributor.authorSze, Heven
dc.contributor.authorPalanivelu, Ravishankar
dc.contributor.authorHarper, Jeffrey F
dc.contributor.authorJohnson, Mark A
dc.date.accessioned2022-01-13T20:27:28Z
dc.date.available2022-01-13T20:27:28Z
dc.date.issued2021-10-02
dc.identifier.citationSze, H., Palanivelu, R., Harper, J. F., & Johnson, M. A. (2021). Holistic insights from pollen omics: Co-opting stress-responsive genes and ER-mediated proteostasis for male fertility. Plant Physiology.en_US
dc.identifier.issn0032-0889
dc.identifier.doi10.1093/plphys/kiab463
dc.identifier.urihttp://hdl.handle.net/10150/662890
dc.description.abstractSexual reproduction in flowering plants takes place without an aqueous environment. Sperm are carried by pollen through air to reach the female gametophyte, though the molecular basis underlying the protective strategy of the male gametophyte is poorly understood. Here we compared the published transcriptomes of Arabidopsis thaliana pollen, and of heat-responsive genes, and uncovered insights into how mature pollen (MP) tolerates desiccation, while developing and germinating pollen are vulnerable to heat stress. Germinating pollen expresses molecular chaperones or “heat shock proteins” in the absence of heat stress. Furthermore, pollen tubes that grew through pistils at basal temperature showed induction of the endoplasmic reticulum (ER) stress response, which is a characteristic of stressed vegetative tissues. Recent studies show MP contains mRNA-protein (mRNP) aggregates that resemble “stress” granules triggered by heat or other stresses to protect cells. Based on these observations, we postulate that mRNP particles are formed in maturing pollen in response to developmentally programmed dehydration. Dry pollen can withstand harsh conditions as it is dispersed in air. We propose that, when pollen lands on a compatible pistil and hydrates, mRNAs stored in particles are released, aided by molecular chaperones, to become translationally active. Pollen responds to osmotic, mechanical, oxidative, and peptide cues that promote ER-mediated proteostasis and membrane trafficking for tube growth and sperm discharge. Unlike vegetative tissues, pollen depends on stress-protection strategies for its normal development and function. Thus, heat stress during reproduction likely triggers changes that interfere with the normal pollen responses, thereby compromising male fertility. This holistic perspective provides a framework to understand the basis of heat-tolerant strains in the reproduction of crops.en_US
dc.description.sponsorshipNational Science Foundation Arabidopsisen_US
dc.language.isoenen_US
dc.publisherOxford University Press (OUP)en_US
dc.rights© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.titleHolistic insights from pollen omics: co-opting stress-responsive genes and ER-mediated proteostasis for male fertilityen_US
dc.typeArticleen_US
dc.identifier.eissn1532-2548
dc.contributor.departmentSchool of Plant Sciences, University of Arizonaen_US
dc.identifier.journalPlant Physiologyen_US
dc.description.noteOpen access articleen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitlePlant Physiology
dc.source.volume187
dc.source.issue4
dc.source.beginpage2361
dc.source.endpage2380
refterms.dateFOA2022-01-13T20:27:29Z


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© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/).