AdvisorRogers, Gregory C.
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PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractCentrosome loss has recently been reported as a phenotype of prostate cancer. Hypoxia, an environmental condition seen commonly in prostate cancer, can cause centrosome loss in the immortalized prostate epithelial cell line, RWPE1. Little is known about hypoxia-induced centrosome loss, including how commonly it occurs in other cell types and the mechanism behind centrosome loss. This thesis further characterizes hypoxia-induced centrosome loss as seen in RWPE1 cells as well as in two other epithelial cell lines, MCF10A and HaCaT. Hypoxia-induced centrosome loss is affected by cell density and is reversible upon return to oxygen in some cell lines. Disassembly of centrosomes in hypoxia may happen through a two-step process, first with the removal of pericentriolar material and then this the disassembly of centrioles.
Degree ProgramGraduate College