Analysis of Position Effect Variegation Using the Switch Monitoring System in Drosophila melanogaster
Publisher
The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
The work outlined throughout the following chapters was completed from December 2016 to October 2021 and examines Position Effect Variegation (PEV) using the Switch Monitoring (SwiM) system. PEV is a phenomenon of heterochromatin biology in which genetically identical cells exhibit differing expression states of euchromatic genes juxtaposed to heterochromatin. The study of PEV has been reliant on the final outcome of these expression states as limitations in scientific observation have prevented a more detailed analysis of the mechanism behind this final pattern. My graduate work utilized a novel fluorescence reporting system, the SwiM system, that allowed us to record and analyze PEV throughout the development of Drosophila melanogaster. The results reported here inform our understanding of the natural fluctuations in heterochromatic silencing that leads to PEV.Type
textElectronic Dissertation
Degree Name
Ph.D.Degree Level
doctoralDegree Program
Graduate CollegeCellular & Molecular Medicine