Teaching the Central Dogma through an Inquiry-Based Project Using GFP
AffiliationDepartment of Molecular and Cellular Biology, University of Arizona
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CitationBujanda, C., & Anderson, N. (2022). Teaching the Central Dogma through an Inquiry-Based Project Using GFP. American Biology Teacher.
JournalAmerican Biology Teacher
RightsCopyright © 2022 by The Regents of the University of California.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThe Central Dogma is a crucial concept needed to understand biotechnology and molecular biology. High school students often struggle with a meaningful understanding of this abstract concept. This paper presents an inquiry-based approach to increase critical thinking and understanding of the Central Dogma. Commonly used in high school classrooms is Bio-Rad pGLO plasmid containing green fluorescent protein (GFP), because of its accessibility and the fluorescence it emits when exposed to ultraviolet light. We use the expression of GFP in a high school hands-on class project so that students can visualize and understand the abstract concepts of the Central Dogma. Students will also explore protein structure and its importance for a functional protein. During the entire project, students will be guided by the instructor to build hypotheses and design experiments to test those hypotheses, exercising the scientific method. ©
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