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dc.contributor.authorDeng, Zhongyuan
dc.contributor.authorZhang, Yuting
dc.contributor.authorGao, Chao
dc.contributor.authorShen, Wei
dc.contributor.authorWang, Shan
dc.contributor.authorNi, Xinzhi
dc.contributor.authorLiu, Sisi
dc.contributor.authorLi, Xianchun
dc.date.accessioned2022-05-18T00:18:58Z
dc.date.available2022-05-18T00:18:58Z
dc.date.issued2022-04-05
dc.identifier.citationDeng, Z., Zhang, Y., Gao, C., Shen, W., Wang, S., Ni, X., Liu, S., & Li, X. (2022). A transposon-introduced G-quadruplex motif is selectively retained and constrained to downregulate CYP321A1. Insect Science.en_US
dc.identifier.issn1672-9609
dc.identifier.doi10.1111/1744-7917.13021
dc.identifier.urihttp://hdl.handle.net/10150/664231
dc.description.abstractInsects utilize xenobiotic compounds to up- and downregulate cytochrome P450 monooxygenases (P450s) involved in detoxification of toxic xenobiotics including phytochemicals and pesticides. G-quadruplexes (G4)-forming DNA motifs are enriched in the promoter regions of transcription factors and function as cis-acting elements to regulate these genes. Whether and how P450s gain and keep G4 DNA motifs to regulate their expression still remain unexplored. Here, we show that CYP321A1, a xenobiotic-metabolizing P450 from Helicoverpa zea, a polyphagous insect of economic importance, has acquired and preserved a G4 DNA motif by selectively retaining a transposon known as HzIS1-3 that carries this G4 DNA motif in its promoter region. The HzIS1-3 G4 DNA motif acts as a silencer to suppress the constitutive and induced expression of CYP321A1 by plant allelochemicals flavone and xanthotoxin through folding into an intramolecular parallel or hybrid-1 conformation in the absence or presence of K+. The G4 ligand N-methylmesoporphyrin IX (NMM) strengthens the silencing effect of HzIS1-3 G4 DNA motif by switching its structure from hybrid-1 to hybrid-2. The enrichment of transposons in P450s and other environment-adaptation genes implies that selective retention of G4 DNA motif-carrying transposons may be the main evolutionary route for these genes to obtain G4 DNA motifs.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2022 Institute of Zoology, Chinese Academy of Sciences.en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.subjectDNA secondary structureen_US
dc.subjectgene regulationen_US
dc.subjectHelicoverpa zeaen_US
dc.subjectnatural selectionen_US
dc.subjectplant allelochemicalsen_US
dc.subjectsignaling pathwayen_US
dc.subjectsilenceren_US
dc.titleA transposon‐introduced G‐quadruplex motif is selectively retained and constrained to downregulate CYP321A1en_US
dc.typeArticleen_US
dc.identifier.eissn1744-7917
dc.contributor.departmentDepartment of Entomology, University of Arizonaen_US
dc.contributor.departmentBIO5 Institute, University of Arizonaen_US
dc.identifier.journalInsect Scienceen_US
dc.description.note12 month embargo; first published: 28 February 2022en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.identifier.pii10.1111/1744-7917.13021
dc.source.journaltitleInsect Science


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