A New Way of Fabricating High-Porosity Parylene Membranes for High-Throughput Capturing of Viable Circulating and Exfoliated Tumor Cells from Large-Volume Bodily Fluids
AuthorRabadi, Inad S.
KeywordsCirculating Tumor Cells
Simple Parylene-C etching
Size-based cell separation
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractWe report a simple method using just photolithography and reactive ion etching to fabricate high porosity Parylene-C porous membranes to process large volume of samples for liquid biopsy. A range of membranes have been fabricated with porosity ranging from 39 to 76 %. In comparison, track-etched polycarbonate membrane has a 5% porosity, and previously reported Parylene membrane had a ~ 45% porosity. A surprising vertical sidewall was observed in etched Parylene pores, which was contributed to the photoresist melting during high DC bias (420V) plasma etching. A circulating tumor cell (CTC) capture device was developed, and buffer sample processing rate up to 200 ml/min (limited by the device’s geometry) was obtained for membranes with porosity of > 47%. A low cell count method with ±1 cell accuracy was developed to characterize the spiked cell capture. The device showed high capture efficiency of 87 to 92% for low spiked cell numbers (< 50 cells). Preliminary results also showed over 90% viability for the captured cells. The simple Parylene etching process is promising for low cost fabrication of high porosity Parylene membranes for liquid biopsy applications.
Degree ProgramGraduate College
Clinical Translational Sciences