Sex‐specific markers undetected in green sunfish Lepomis cyanellus using restriction‐site associated DNA sequencing
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Genetic Sex ID markers in ...
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Final Accepted Manuscript
Author
Teal, Chad N.Coykendall, D. Katharine
Campbell, Matthew R.
Eardley, Daniel L.
Delomas, Thomas A.
Shira, James T.
Schill, Daniel J.
Bonar, Scott A.
Culver, Melanie
Affiliation
University of Arizona Genetics CoreSchool of Natural Resources and the Environment, University of Arizona
Issue Date
2022-05-06
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WileyCitation
Teal, C. N., Coykendall, D. K., Campbell, M. R., Eardley, D. L., Delomas, T. A., Shira, J. T., Schill, D. J., Bonar, S. A., & Culver, M. (2022). Sex-specific markers undetected in green sunfish Lepomis cyanellus using restriction-site associated DNA sequencing. Journal of Fish Biology.Journal
Journal of Fish BiologyRights
© 2022 Fisheries Society of the British Isles.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
We used restriction-site associated DNA sequencing for SNP discovery and genotyping of known-sex green sunfish Lepomis cyanellus DNA samples to search for sex-diagnostic single nucleotide polymorphisms (SNPs) and restriction-site associated sequences present in one sex and absent in the other. The bioinformatic analyses discovered candidate SNPs and sex-specific restriction-site associated sequences that fit patterns of male or female heterogametic sex determination systems. However, when primers were developed and tested, no candidates reliably identified phenotypic sex. The top performing SNP candidate (ZW_218) correlated with phenotypic sex 63.0% of the time and the presence-absence loci universally amplified in both sexes. We recommend further investigations that interrogate a larger fraction of the L. cyanellus genome. Additionally, studies on the effect of temperature and rearing density on sex determination, as well as breeding of sex-reversed individuals, could provide more insights into the sex determination system of L. cyanellus.Note
12 month embargo; first published: 19 April 2022ISSN
0022-1112EISSN
1095-8649Version
Final accepted manuscriptae974a485f413a2113503eed53cd6c53
10.1111/jfb.15063