Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

Hill, S.M.; Padilla-Rodriguez, M.; Clements, A.; Sweetland, J.A.; Parker, S.S.; Warfel, N.A.; Mouneimne, G.

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Author

Hill, S.M.
Padilla-Rodriguez, M.
Clements, A.
Sweetland, J.A.
Parker, S.S.
Warfel, N.A.
Mouneimne, G.

Affiliation

Department of Cancer Biology GIDP, University of Arizona
Department of Cellular and Molecular Medicine, University of Arizona
Department of Science, University of Arizona

Issue Date

2022

Citation

Hill, S. M., Padilla-Rodriguez, M., Clements, A., Sweetland, J. A., Parker, S. S., Warfel, N. A., & Mouneimne, G. (2022). Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior. STAR Protocols, 3(3).

Journal

STAR Protocols

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This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.

Abstract

We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021). © 2022 The Author(s)

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Open access journal

ISSN

2666-1667

DOI

10.1016/j.xpro.2022.101516

Version

Final published version

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UA Faculty Publications

Copyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Except where otherwise noted, this item's license is described as Copyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).