Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior
AffiliationDepartment of Cancer Biology GIDP, University of Arizona
Department of Cellular and Molecular Medicine, University of Arizona
Department of Science, University of Arizona
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CitationHill, S. M., Padilla-Rodriguez, M., Clements, A., Sweetland, J. A., Parker, S. S., Warfel, N. A., & Mouneimne, G. (2022). Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior. STAR Protocols, 3(3).
RightsCopyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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AbstractWe describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021). © 2022 The Author(s)
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Except where otherwise noted, this item's license is described as Copyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).