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Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

dc.contributor.authorHill, S.M.
dc.contributor.authorPadilla-Rodriguez, M.
dc.contributor.authorClements, A.
dc.contributor.authorSweetland, J.A.
dc.contributor.authorParker, S.S.
dc.contributor.authorWarfel, N.A.
dc.contributor.authorMouneimne, G.
dc.date.accessioned2022-08-01T20:21:24Z
dc.date.available2022-08-01T20:21:24Z
dc.date.issued2022
dc.identifier.citationHill, S. M., Padilla-Rodriguez, M., Clements, A., Sweetland, J. A., Parker, S. S., Warfel, N. A., & Mouneimne, G. (2022). Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior. STAR Protocols, 3(3).
dc.identifier.issn2666-1667
dc.identifier.doi10.1016/j.xpro.2022.101516
dc.identifier.urihttp://hdl.handle.net/10150/665522
dc.description.abstractWe describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021). © 2022 The Author(s)
dc.language.isoen
dc.publisherCell Press
dc.rightsCopyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectBiophysics
dc.subjectCancer
dc.subjectCell Biology
dc.titleOptimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior
dc.typeArticle
dc.typetext
dc.contributor.departmentDepartment of Cancer Biology GIDP, University of Arizona
dc.contributor.departmentDepartment of Cellular and Molecular Medicine, University of Arizona
dc.contributor.departmentDepartment of Science, University of Arizona
dc.identifier.journalSTAR Protocols
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleSTAR Protocols
refterms.dateFOA2022-08-01T20:21:24Z


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Copyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).