IDENTIFICATION OF NOVEL CHANGES IN C2C12 MYOTUBE PROTEOME AFTER ELECTRICAL PULSE STIMULATION

Abstract

Type 2 diabetes mellitus (T2DM) and obesity are common and complex metabolic diseases with high prevalence rates across the United States and worldwide. Exercise is a first-line treatment for T2DM and obesity. Many human and mouse model investigations have been performed to determine the molecular mechanisms that explain how exercise improves skeletal muscle metabolism in these diseases. However, in vivo exercise models are often very time-consuming, costly, and have limitations on the experiments that can be performed, especially in human subjects such as gene knockout experiments. Electrical pulse stimulation (EPS) has been increasingly studied in recent years as an in vitro alternative to exercise interventions in human and mouse models. This paper describes how we set up EPS as an in vitro equivalent to exercise within our laboratory, and we describe and identify proteomic changes in the C2C12 murine myotubes following EPS.