STRESS ALTERS THE EXPRESSION OF RASPUTIN, A CRITICAL STRESS GRANULE COMPONENT IN AGING FRUIT FLY
AuthorTran, My Duyen Luong
AdvisorZarnescu, Daniela C.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractRNA stress granules (SGs) are non-membranous organelles that condense during cellular stress, such as oxidative and osmotic pressure, and inhibited translation. Depending on the cell types and stress, SGs sequester translation initiation factors and non-translating RNA/protein complexes. SGs have been proposed to modulate signaling pathways in normal stress response, and shown to increase fitness, protect RNA from damage, and delay to aggregation of proteins linked to neurodegeneration during stress. However, dysregulation of SG formation and clearance has been linked to neurodegenerative diseases and is also disrupted in aging. Because age is the biggest risk factor in neurodegeneration, we are interested in how SG dynamics are affected in aging. To better understand and characterize this process, we visualized changes in SGs during aging and stress by imaging the brains of adult flies (Drosophila melanogaster) at different ages, young (1-day), middle-aged (30-days), and old (60-days). The Drosophila line used for this experiment, Rin-GFP and mth1-Rin-GFP, harbors a GFP tag at the C terminus of Rasputin (Rin), the Drosophila homolog of G3BP1, which is critical for SG formation. Our results showed that mth1 and mth1-Rin-GFP are indeed more resilient to stress and that SG assembly in flies vary by age and upon stress. In aging, varying dynamics could contribute to decrease stress resiliency. Uncovering dynamic alterations in SGs upon stress could provide insights into the mechanism of stress resilience at the molecular level.
Degree ProgramMolecular and Cellular Biology