Depth random-access two-photon Bessel light-sheet imaging in brain tissue
AffiliationCollege of Optical Science, University of Arizona
MetadataShow full item record
PublisherOptica Publishing Group (formerly OSA)
CitationXu, D., Ding, J. B., & Peng, L. (2022). Depth random-access two-photon Bessel light-sheet imaging in brain tissue. Optics Express, 30(15), 26396–26406.
RightsCopyright © 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractTwo-photon light-sheet fluorescence microscopy enables high-resolution imaging of neural activity in brain tissue at a high frame rate. Traditionally, light-sheet microscopy builds up a 3D stack by multiple depth scans with uniform spatial intervals, which substantially limits the volumetric imaging speed. Here, we introduce the depth random-access light-sheet microscopy, allowing rapid switching scanning depth for light-sheet imaging. With a low-cost electrically tunable lens and minimum modification of an existing two-photon light-sheet imaging instrument, we demonstrated fast random depth hopping light-sheet imaging at 100 frames per second in the live brain slice. Through depth random-access, calcium activities for an astrocyte were recorded on four user-selected detection planes at a refreshing rate of 25 Hz. © 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
NoteOpen access journal
VersionFinal published version