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    Investigating Peptide-Lipid Interactions in Nanodiscs Using Native Mass Spectrometry and Fast Photochemical Oxidation of Peptides

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    azu_etd_20012_sip1_m.pdf
    Embargo:
    2023-10-12
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    Author
    Reid, Deseree JoAnne
    Issue Date
    2022
    Keywords
    Antimicrobial peptides
    FPOP
    Nanodiscs
    Advisor
    Marty, Michael
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Embargo
    Release after 10/12/2023
    Abstract
    Antimicrobial peptides (AMPs) are an important part of the innate immune system and demonstrate promising applications in the fight against antibiotic resistant infections due to their unique mechanism of targeting bacterial membranes. Nevertheless, it is challenging to study the interactions of these peptides within lipid bilayers, making it difficult to understand their mechanisms of toxicity and selectivity. Lipoprotein nanodiscs are ideally suited for the study of AMPs with native mass spectrometry because they provide a relatively monodisperse nanoscale lipid bilayer environment for delivering membrane peptides into the gas phase. However, native mass spectrometry of nanodiscs produces complex spectra that can be challenging to assign unambiguously. This dissertation first describes a method to simplify interpretation of nanodisc spectra using an engineered series of mutant membrane scaffold proteins (MSP) that do not affect nanodisc formation but shift the masses of nanodiscs in a controllable way, thus eliminating isobaric interference from the lipids. Moreover, by mixing two different belts before assembly, the stoichiometry of MSP is encoded in the peak shape, which allows the stoichiometry to be assigned unambiguously from a single spectrum. Next, this dissertation describes the use of fast photochemical oxidation of peptides (FPOP), an irreversible footprinting technique that labels solvent accessible residues, in combination with LC-MS-MS and native charge detection-mass spectrometry to study AMP-lipid interactions and surface exposed residues within different lipid bilayer nanodiscs. The findings herein provide complementary information on the potential modes of action and lipid selectivity of various AMPs.
    Type
    text
    Electronic Dissertation
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Graduate College
    Chemistry
    Degree Grantor
    University of Arizona
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    Dissertations

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