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dc.contributor.authorMashaqi, S.
dc.contributor.authorLaubitz, D.
dc.contributor.authorMorales, E.J.D.
dc.contributor.authorDe Armond, R.
dc.contributor.authorAlameddin, H.
dc.contributor.authorGhishan, F.K.
dc.contributor.authorKiela, P.R.
dc.contributor.authorParthasarathy, S.
dc.date.accessioned2022-10-24T23:51:25Z
dc.date.available2022-10-24T23:51:25Z
dc.date.issued2022
dc.identifier.citationMashaqi, S., Laubitz, D., Morales, E. J. D., De Armond, R., Alameddin, H., Ghishan, F. K., Kiela, P. R., & Parthasarathy, S. (2022). Interactive Effect of Combined Intermittent and Sustained Hypoxia and High-Fat Diet on the Colonic Mucosal Microbiome and Host Gene Expression in Mice. Nature and Science of Sleep, 14, 1623–1639.
dc.identifier.issn1179-1608
dc.identifier.doi10.2147/NSS.S370957
dc.identifier.urihttp://hdl.handle.net/10150/666489
dc.description.abstractPurpose: Gut dysbiosis can cause cardiometabolic disease. Gut dysbiosis can be independently caused by high-fat diet (HFD) and intermittent hypoxia (IH; characterizing obstructive sleep apnea), but the interactive effect of combined intermittent and sustained hypoxia (IH+SH) (characterizing obesity hypoventilation syndrome) and HFD on gut dysbiosis is unclear. We aimed to investigate the interactive effect of a combination of IH and SH and HFD on proximal colonic microbiota and colonic gene expression pattern. Methods: Male mice (n=16) were randomly received four different combinations of diet (normal versus HFD) and oxygen conditions (normoxia versus IH+SH) for 4 weeks. Bacterial DNA and mucosal epithelial cell RNA from proximal colon were collected for analysis of adherent microbiome and host’s gene expression analysis. Results: HFD during IH+SH (22.6 ± 5.73; SD) led to greater Firmicutes: Bacteroidetes ratio than HFD during normoxia (5.89 ± 1.19; p=0.029). HFD significantly decreased microbial diversity as compared to normal diet, but the addition of IH+SH to HFD mildly reversed such effects. When compared to HFD during normoxia, HFD with combination of IH+SH resulted in changes to host mucosal gene expression for apical junctional complexes and adhesion molecules. Specifically, when compared to HFD during normoxia, HFD during IH+SH led to upregulation of Claudin 2 and Syk (tight junction dysfunction and increased mucosal permeability), while the barrier promoting claudin 4 was downregulated. Conclusion: HFD during combined IH and SH causes greater gut dysbiosis and potentially adverse changes in colonic epithelial transcriptome than HFD during normoxia. The latter changes are suggestive of impaired gut barrier function. © 2022 Mashaqi et al.
dc.language.isoen
dc.publisherDove Medical Press Ltd
dc.rightsCopyright © 2022 Mashaqi et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/).
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.titleInteractive Effect of Combined Intermittent and Sustained Hypoxia and High-Fat Diet on the Colonic Mucosal Microbiome and Host Gene Expression in Mice
dc.typeArticle
dc.typetext
dc.contributor.departmentDepartment of Pulmonary, Allergy, Critical Care, and Sleep, University of Arizona College of Medicine
dc.contributor.departmentUniversity of Arizona Health Sciences Center for Sleep & Circadian Sciences, University of Arizona College of Medicine
dc.contributor.departmentDepartment of Pediatrics, Steele Children’s Research Center, University of Arizona College of Medicine
dc.contributor.departmentUniversity of Arizona College of Pharmacy
dc.contributor.departmentDepartment of Immunobiology, University of Arizona College of Medicine
dc.identifier.journalNature and Science of Sleep
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleNature and Science of Sleep
refterms.dateFOA2022-10-24T23:51:25Z


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Copyright © 2022 Mashaqi et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/).
Except where otherwise noted, this item's license is described as Copyright © 2022 Mashaqi et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/).