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dc.contributor.authorWidyawan, A.
dc.contributor.authorIbrahim, Y.E.
dc.contributor.authorKomy, M.H.E.
dc.contributor.authorAl Dhafer, H.M.
dc.contributor.authorBrown, J.K.
dc.contributor.authorAl-Saleh, M.A.
dc.date.accessioned2022-12-15T22:41:21Z
dc.date.available2022-12-15T22:41:21Z
dc.date.issued2023
dc.identifier.citationWidyawan, A., Ibrahim, Y. E., Komy, M. H. E., Al Dhafer, H. M., Brown, J. K., & Al-Saleh, M. A. (2023). Differentiation of ‘Candidatus Liberibacter asiaticus’ in Saudi Arabia based on tandem repeat variability in genomic locus. Journal of King Saud University - Science, 35(1).
dc.identifier.issn1018-3647
dc.identifier.doi10.1016/j.jksus.2022.102376
dc.identifier.urihttp://hdl.handle.net/10150/667214
dc.description.abstractCitrus greening, or huanglongbing, is a destructive disease threatening many citrus worldwide, and drastically altering the global dynamics of the citrus industry. The disease is caused by one of several unculturable bacterial species belonging to ‘Candidatus Liberibacter’. The recent availability of complete genome sequences of ‘Candidatus Liberibacter asiaticus’ (CLas) has facilitated comprehensive assessments of genomic variability using a range of approaches, including short tandem repeat analysis. The objective of this study was to evaluate the genetic diversity of CLas populations in Saudi Arabia based on tandem repeat number (TRN) within the CLIBASIA_01645 locus, predicted to encode the bacteriophage C1 repressor protein. Results indicated that the genotype richness of the Saudi Arabian CLas isolates was conserved by 27% based on the TRN locus. Four different genotypes TRN2, TRN3, TRN4, and TRN5 were identified. However, the TRN2 and TRN5 were the most dominant genotypes. All four of the TRN genotypes were associated with CLas-positive mandarin (Citrus reticulata) or sweet orange (C. sinensis) citrus trees. The diversity (H = 0.69) and evenness (H'=0.914) were overall relatively high, with the northern region of Saudi Arabia harboring the highest diversity (0.7) and evenness score (0.9–1.0). Phylogenetic analysis of the CLas-bacteriophage C1 repressor protein of the Saudi Arabian isolates indicated CLas was more closely related to ‘Candidatus Liberibacter africanus’ than to ‘Candidatus Liberibacter americanus’. © 2022 The Authors
dc.language.isoen
dc.publisherElsevier B.V.
dc.rightsCopyright © 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBacteriophage C1 repressor protein
dc.subjectCitrus
dc.subjectGenetic diversity
dc.subjectHuanglongbing
dc.subjectMinisatellite
dc.titleDifferentiation of ‘Candidatus Liberibacter asiaticus’ in Saudi Arabia based on tandem repeat variability in genomic locus
dc.typeArticle
dc.typetext
dc.contributor.departmentSchool of Plant Sciences, University of Arizona
dc.identifier.journalJournal of King Saud University - Science
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleJournal of King Saud University - Science
refterms.dateFOA2022-12-15T22:41:21Z


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Copyright © 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as Copyright © 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).