Airway basal cells show regionally distinct potential to undergo metaplastic differentiation
Affiliation
Department of Pharmacy Practice and Science, College of Pharmacy, University of ArizonaIssue Date
2022Keywords
airway epitheliumbasal cell heterogeneity
developmental biology
human
lung repair
mouse
organotypic cultures
regenerative medicine
stem cell
stem cells
Metadata
Show full item recordPublisher
eLife Sciences PublicationsCitation
Zhou, Y., Yang, Y., Guo, L., Qian, J., Ge, J., Sinner, D., Ding, H., Califano, A., & Cardoso, W. V. (2022). Airway basal cells show regionally distinct potential to undergo metaplastic differentiation. ELife, 11.Journal
eLifeRights
Copyright © Zhou, Yang, Guo et al. This article is distributed under the terms of the Creative Commons Attribution License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Basal cells are multipotent stem cells of a variety of organs, including the respiratory tract, where they are major components of the airway epithelium. However, it remains unclear how diverse basal cells are and how distinct subpopulations respond to airway challenges. Using single cell RNA-sequencing and functional approaches, we report a significant and previously underappreciated degree of heterogeneity in the basal cell pool, leading to identification of six subpopulations in the adult murine trachea. Among these, we found two major subpopulations, collectively comprising the most uncommitted of all the pools, but with distinct gene expression signatures. Notably, these occupy distinct ventral and dorsal tracheal niches and differ in their ability to self-renew and initiate a program of differentiation in response to environmental perturbations in primary cultures and in mouse injury models in vivo. We found that such heterogeneity is acquired prenatally, when the basal cell pool and local niches are still being established, and depends on the integrity of these niches, as supported by the altered basal cell phenotype of tracheal cartilage-deficient mouse mutants. Finally, we show that features that distinguish these progenitor subpopulations in murine airways are conserved in humans. Together, the data provide novel insights into the origin and impact of basal cell heterogeneity on the establishment of regionally distinct responses of the airway epithelium during injury-repair and in disease conditions. © 2022, Zhou, Yang, Guo et al.Note
Open access journalISSN
2050-084XPubMed ID
36178196Version
Final published versionae974a485f413a2113503eed53cd6c53
10.7554/eLife.80083
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Except where otherwise noted, this item's license is described as Copyright © Zhou, Yang, Guo et al. This article is distributed under the terms of the Creative Commons Attribution License.
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